Todd A J
Department of Anatomy, University of Glasgow, U.K.
Neuroscience. 1991;44(3):741-6. doi: 10.1016/0306-4522(91)90093-4.
Pre-embedding immunohistochemistry with monoclonal antibody to choline acetyltransferase was combined with post-embedding immunohistochemistry with antisera to GABA and glycine in order to study the pattern of coexistence of GABA, glycine and acetylcholine in neurons in lamina III of rat spinal dorsal horn. Of 50 neurons which were choline acetyltransferase immunoreactive, 47 showed GABA-like immunoreactivity and none were immunoreactive with antiserum to glycine, despite the fact that glycine is thought to be present in the majority of GABAergic neurons in lamina III. This suggests that while acetylcholine and glycine can both coexist with GABA in lamina III neurons, they are present in different populations of GABAergic cells. Taken together with recent ultrastructural evidence concerning the synaptic connections of glycinergic and cholinergic structures in the dorsal horn, this suggests that there are functional differences between neurons which contain GABA and glycine and those which contain GABA and acetylcholine.
为了研究大鼠脊髓背角Ⅲ层神经元中γ-氨基丁酸（GABA）、甘氨酸和乙酰胆碱的共存模式，将用抗胆碱乙酰转移酶单克隆抗体进行的包埋前免疫组织化学与用抗GABA和甘氨酸抗血清进行的包埋后免疫组织化学相结合。在50个具有胆碱乙酰转移酶免疫反应性的神经元中，47个显示出GABA样免疫反应性，没有一个与甘氨酸抗血清发生免疫反应，尽管据认为甘氨酸存在于Ⅲ层的大多数GABA能神经元中。这表明，虽然乙酰胆碱和甘氨酸都可以与GABA共存于Ⅲ层神经元中，但它们存在于不同的GABA能细胞群体中。结合最近关于背角中甘氨酸能和胆碱能结构突触连接的超微结构证据，这表明含有GABA和甘氨酸的神经元与含有GABA和乙酰胆碱的神经元之间存在功能差异。
