Reynolds E S, Moslen M T, Szabo S, Jaeger R J
Res Commun Chem Pathol Pharmacol. 1975 Dec;12(4):685-94.
Liver microsomal enzyme activities were measured 24 hr after a 6 hr exposure to 5% vinyl chloride in male rats pretreated with phenobarbital, Aroclor 1254 or the control vehicle. Liver injury as indicated by serum transaminase elevations after vinyl chloride, was found only in phenobarbital and Aroclor 1254 animals while cytochrome P-450 contents and oxidative N-demethylation of aminoantipyrine and ethylmorphine were markedly decreased in all groups. Glucose-6-phosphatase, cytochrome b5 and NADPH and NADH cytochrome P-450 reductase were scarcely affected. This selective enzymatic deactivation is consistent with a cytochrome P-450 centered activation of vinyl chloride to a reactive electrophile.
在用苯巴比妥、多氯联苯混合物1254或对照赋形剂预处理的雄性大鼠中，暴露于5%氯乙烯6小时后24小时测量肝脏微粒体酶活性。氯乙烯暴露后血清转氨酶升高所表明的肝损伤仅在苯巴比妥和多氯联苯混合物1254处理的动物中发现，而所有组中细胞色素P-450含量以及氨基安替比林和乙基吗啡的氧化N-脱甲基作用均显著降低。葡萄糖-6-磷酸酶、细胞色素b5以及NADPH和NADH细胞色素P-450还原酶几乎未受影响。这种选择性酶失活与以细胞色素P-450为中心的氯乙烯激活为活性亲电试剂相一致。
