García-Godoy Franklin, Tay Franklin R, Pashley David H, Feilzer Albert, Tjäderhane L, Pashley Edna L
Bioscience Research Center, College of Dental Medicine, Nova Southeastern University, 3200 South University Drive, Fort Lauderdale, Florida 33328, USA.
Am J Dent. 2007 Apr;20(2):109-13.
To test, in vitro, the null hypothesis that there was no difference in the ultrastructure of adhesive-bonded, acid-etched dentin aged under accelerated conditions in mineral oil or artificial saliva.
Beams of human dentin bonded with three total-etch adhesives were retrieved from the two storage media after 3 years and prepared for transmission electron microscopy.
Hybrid layers from specimens aged in mineral oil exhibited structural integrity of the collagen network. Conversely, abnormal hybrid layers were seen in specimens aged in artificial saliva, with progressive disintegration of the fibrillar network to the extent that it was beyond detection by collagen staining. Self-destruction of collagen matrices can occur in resin-infiltrated dentin, and to a lesser degree, in mineralized dentin in the absence of bacterial or salivary enzymes.
在体外检验以下零假设，即在矿物油或人工唾液中加速老化的酸蚀牙本质与粘结牙本质的超微结构不存在差异。
使用三种全酸蚀粘结剂粘结的人牙本质梁在两种储存介质中保存3年后取出，制备用于透射电子显微镜检查的样本。
在矿物油中老化的样本的混合层显示出胶原网络的结构完整性。相反，在人工唾液中老化的样本中观察到异常的混合层，纤维状网络逐渐解体，以至于胶原染色无法检测到。在没有细菌或唾液酶的情况下，树脂浸润的牙本质中的胶原基质会发生自毁，在矿化牙本质中自毁程度较小。
