Corporate Affiliated Research Institute, Feagle Co., Ltd., Yangsan 50561, Republic of Korea.
Department of Dental Hygiene, Kyungnam College of Information & Technology, Busan 47011, Republic of Korea.
Medicina (Kaunas). 2024 Aug 14;60(8):1318. doi: 10.3390/medicina60081318.
: Enhanced osteoblast differentiation may be leveraged to prevent and treat bone-related diseases such as osteoporosis. No-ozone cold plasma (NCP) treatment is a promising and safe strategy to enhance osteoblast differentiation. Therefore, this study aimed to determine the effectiveness of direct and indirect NCP treatment methods on osteoblast differentiation. Mouse osteoblastic cells (MC3T3-E1) were treated with NCP using different methods, i.e., no NCP treatment (NT group; control), direct NCP treatment (DT group), direct NCP treatment followed by media replacement (MC group), and indirect treatment with NCP-treated media only (PAM group). : The MC3T3-E1 cells were subsequently assessed for cell proliferation, alkaline phosphatase (ALP) activity, calcium deposition, and ALP and osteocalcin mRNA expression using real-time polymerase chain reaction. : Cell proliferation significantly increased in the NCP-treated groups (DT and PAM; MC and PAM) compared to the NT group after 24 h ( < 0.038) and 48 h ( < 0.000). ALP activity was increased in the DT and PAM groups at 1 week ( < 0.115) and in the DT, MC, and PAM groups at 2 weeks ( < 0.000) compared to the NT group. Calcium deposition was higher in the NCP-treated groups than in NT group at 2 and 3 weeks ( < 0.000). ALP mRNA expression peaked in the MC group at 2 weeks compared to the NP group ( < 0.014). Osteocalcin mRNA expression increased in the MC group at 2 weeks ( < 0.000) and was the highest in the PAM group at 3 weeks ( < 0.000). Thus, the effects of direct (DT and MC) and indirect (PAM) treatment varied, with MC direct treatment showing the most significant impact on osteoblast activity. : The MC group exhibited enhanced osteoblast differentiation, indicating that direct NCP treatment followed by media replacement is the most effective method for promoting bone formation.
增强成骨细胞分化可能被利用来预防和治疗与骨骼相关的疾病，如骨质疏松症。无臭氧冷等离子体（NCP）治疗是一种有前途且安全的策略，可以增强成骨细胞分化。因此，本研究旨在确定直接和间接 NCP 处理方法对成骨细胞分化的有效性。使用不同方法用 NCP 处理小鼠成骨细胞（MC3T3-E1），即无 NCP 处理（NT 组；对照）、直接 NCP 处理（DT 组）、直接 NCP 处理后更换培养基（MC 组）和仅用 NCP 处理的培养基间接处理（PAM 组）。随后使用实时聚合酶链反应评估 MC3T3-E1 细胞的细胞增殖、碱性磷酸酶（ALP）活性、钙沉积以及 ALP 和骨钙素 mRNA 表达。与 NT 组相比，NCP 处理组（DT 和 PAM；MC 和 PAM）在 24 小时（ < 0.038）和 48 小时（ < 0.000）后细胞增殖显著增加。在 1 周时，DT 和 PAM 组的 ALP 活性增加（ < 0.115），在 2 周时，DT、MC 和 PAM 组的 ALP 活性增加（ < 0.000）与 NT 组相比。在 2 周和 3 周时，NCP 处理组的钙沉积均高于 NT 组（ < 0.000）。与 NP 组相比，MC 组的 ALP mRNA 表达在 2 周时达到峰值（ < 0.014）。在 2 周时，MC 组的骨钙素 mRNA 表达增加（ < 0.000），在 3 周时，PAM 组的骨钙素 mRNA 表达最高（ < 0.000）。因此，直接（DT 和 MC）和间接（PAM）处理的效果不同，MC 直接处理对成骨细胞活性的影响最大。MC 组表现出增强的成骨细胞分化，表明直接 NCP 处理后更换培养基是促进骨形成最有效的方法。
