Bao Yiming, Bolotov Pavel, Dernovoy Dmitry, Kiryutin Boris, Tatusova Tatiana
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W280-4. doi: 10.1093/nar/gkm354. Epub 2007 Jun 1.
FLAN (short for FLu ANnotation), the NCBI web server for genome annotation of influenza virus (http://www.ncbi.nlm.nih.gov/genomes/FLU/Database/annotation.cgi) is a tool for user-provided influenza A virus or influenza B virus sequences. It can validate and predict protein sequences encoded by an input flu sequence. The input sequence is BLASTed against a database containing influenza sequences to determine the virus type (A or B), segment (1 through 8) and subtype for the hemagglutinin and neuraminidase segments of influenza A virus. For each segment/subtype of the viruses, a set of sample protein sequences is maintained. The input sequence is then aligned against the corresponding protein set with a 'Protein to nucleotide alignment tool' (ProSplign). The translated product from the best alignment to the sample protein sequence is used as the predicted protein encoded by the input sequence. The output can be a feature table that can be used for sequence submission to GenBank (by Sequin or tbl2asn), a GenBank flat file, or the predicted protein sequences in FASTA format. A message showing the length of the input sequence, the predicted virus type, segment and subtype for the hemagglutinin and neuraminidase segments of Influenza A virus will also be displayed.
FLAN（流感注释的缩写）是美国国家生物技术信息中心（NCBI）的一个网络服务器，用于流感病毒的基因组注释（http://www.ncbi.nlm.nih.gov/genomes/FLU/Database/annotation.cgi），它是一个供用户提供甲型流感病毒或乙型流感病毒序列的工具。它可以验证并预测输入流感序列所编码的蛋白质序列。输入序列会与一个包含流感序列的数据库进行BLAST比对，以确定病毒类型（A或B）、片段（1至8）以及甲型流感病毒血凝素和神经氨酸酶片段的亚型。对于每种病毒的片段/亚型，都维护着一组样本蛋白质序列。然后，使用“蛋白质到核苷酸比对工具”（ProSplign）将输入序列与相应的蛋白质集进行比对。与样本蛋白质序列最佳比对的翻译产物被用作输入序列所编码的预测蛋白质。输出可以是一个特征表（可用于通过Sequin或tbl2asn向GenBank提交序列）、一个GenBank平面文件，或者是FASTA格式的预测蛋白质序列。还会显示一条消息，表明输入序列的长度、预测的病毒类型、甲型流感病毒血凝素和神经氨酸酶片段的片段及亚型。
