铜绿假单胞菌中parA（soj）同源物的缺失会导致ParB不稳定，并影响生长速率、染色体分离和运动性。
Deletion of the parA (soj) homologue in Pseudomonas aeruginosa causes ParB instability and affects growth rate, chromosome segregation, and motility.
作者信息
Lasocki Krzysztof, Bartosik Aneta A, Mierzejewska Jolanta, Thomas Christopher M, Jagura-Burdzy Grazyna
机构信息
The Institute of Biochemistry and Biophysics, PAS, 02-106 Warsaw, Pawinskiego 5A, Poland.
出版信息
J Bacteriol. 2007 Aug;189(15):5762-72. doi: 10.1128/JB.00371-07. Epub 2007 Jun 1.
Abstract
The parA and parB genes of Pseudomonas aeruginosa are located approximately 8 kb anticlockwise from oriC. ParA is a cytosolic protein present at a level of around 600 molecules per cell in exponential phase, but the level drops about fivefold in stationary phase. Overproduction of full-length ParA or the N-terminal 85 amino acids severely inhibits growth of P. aeruginosa and P. putida. Both inactivation of parA and overexpression of parA in trans in P. aeruginosa also lead to accumulation of anucleate cells and changes in motility. Inactivation of parA also increases the turnover rate (degradation) of ParB. This may provide a mechanism for controlling the level of ParB in response to the growth rate and expression of the parAB operon.
摘要
铜绿假单胞菌的parA和parB基因位于距oriC逆时针方向约8 kb处。ParA是一种胞质蛋白，在指数生长期每个细胞中的含量约为600个分子，但在稳定期该含量下降约五倍。全长ParA或N端85个氨基酸的过量表达会严重抑制铜绿假单胞菌和恶臭假单胞菌的生长。parA的失活以及在铜绿假单胞菌中反式过表达parA也会导致无核细胞的积累和运动性的改变。parA的失活还会增加ParB的周转率（降解）。这可能提供了一种根据生长速率和parAB操纵子的表达来控制ParB水平的机制。
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