Ling Peng, Wang Meinan, Chen Xianming, Campbell Kimberly Garland
US Department of Agriculture, Agricultural Research Service, Wheat Genetic, Quality, Physiology and Disease Research Unit, Pullman, WA 99164-6430, USA.
BMC Genomics. 2007 Jun 4;8:145. doi: 10.1186/1471-2164-8-145.
Puccinia striiformis is a plant pathogenic fungus causing stripe rust, one of the most important diseases on cereal crops and grasses worldwide. However, little is know about its genome and genes involved in the biology and pathogenicity of the pathogen. We initiated the functional genomic research of the fungus by constructing a full-length cDNA and determined functions of the first group of genes by sequence comparison of cDNA clones to genes reported in other fungi.
A full-length cDNA library, consisting of 42,240 clones with an average cDNA insert of 1.9 kb, was constructed using urediniospores of race PST-78 of P. striiformis f. sp. tritici. From 196 sequenced cDNA clones, we determined functions of 73 clones (37.2%). In addition, 36 clones (18.4%) had significant homology to hypothetical proteins, 37 clones (18.9%) had some homology to genes in other fungi, and the remaining 50 clones (25.5%) did not produce any hits. From the 73 clones with functions, we identified 51 different genes encoding protein products that are involved in amino acid metabolism, cell defense, cell cycle, cell signaling, cell structure and growth, energy cycle, lipid and nucleotide metabolism, protein modification, ribosomal protein complex, sugar metabolism, transcription factor, transport metabolism, and virulence/infection.
The full-length cDNA library is useful in identifying functional genes of P. striiformis.
条形柄锈菌是一种引起条锈病的植物病原真菌，条锈病是全球谷类作物和禾本科植物最重要的病害之一。然而，对于其基因组以及参与该病原菌生物学特性和致病性的基因了解甚少。我们通过构建全长cDNA文库启动了该真菌的功能基因组研究，并通过将cDNA克隆与其他真菌中报道的基因进行序列比较来确定第一组基因的功能。
利用小麦条锈菌生理小种PST - 78的夏孢子构建了一个全长cDNA文库，该文库由42,240个克隆组成，平均cDNA插入片段大小为1.9 kb。从196个测序的cDNA克隆中，我们确定了73个克隆（37.2%）的功能。此外，36个克隆（18.4%）与假定蛋白具有显著同源性，37个克隆（18.9%）与其他真菌中的基因有一定同源性，其余50个克隆（25.5%）未产生任何匹配结果。从73个具有功能的克隆中，我们鉴定出51个不同的基因，其编码的蛋白质产物参与氨基酸代谢、细胞防御、细胞周期、细胞信号传导、细胞结构与生长、能量循环、脂质和核苷酸代谢、蛋白质修饰、核糖体蛋白复合体、糖代谢、转录因子、转运代谢以及毒力/感染等过程。
全长cDNA文库有助于鉴定条形柄锈菌的功能基因。
