Albanidou-Farmaki Eleni, Markopoulos Anastasios K, Kalogerakou Filanthi, Antoniades Demetrios Z
Department of Oral Medicine & Maxillofacial Pathology, School of Dentistry, Aristotle University of Thessaloniki, Greece.
Tohoku J Exp Med. 2007 Jun;212(2):101-5. doi: 10.1620/tjem.212.101.
One of the factors involved in the pathogenesis of recurrent aphthous stomatitis (RAS) is a cell-mediated immune response in which several cytokines seem to play a major role. The aim of this study was to detect, enumerate and characterize T helper cells which are secreting type 1 cytokines (interleukin [IL]-2, IL-12, interferon [IFN]-gamma, and tumor necrosis factor [TNF]-alpha) and type 2 cytokines (IL-4, IL-5, IL-6, and IL-10) in the peripheral blood of patients with RAS. Thirty-two patients in the active phase of RAS (14 men and 18 women) and 40 healthy individuals participated in the study. T helper (T) cells were detected and characterized using Elispot assay. T cells secreting IL-2, IL-12 or IFN-gamma were increased in patients with RAS compared with the controls (p < 0.05, p < 0.001 and p < 0.001, respectively). T cells secreting TNF-alpha in RAS patients and controls were not statistically different (p > 0.05). T cells secreting IL-10 were increased in patients with RAS compared with the controls (p < 0.05). T cells secreting IL-4 were decreased in patients with RAS compared with the controls (p < 0.001), No statistical difference was observed between T cells secreting IL-5 or IL-6 in patients with RAS and controls. Our findings suggest that the increased numbers of T cells secreting type 1 cytokines may influence the immune response against RAS. Whether this action is of etiological importance or epigenetic phenomenon is a question that needs to be answered.
复发性阿弗他口炎（RAS）发病机制涉及的因素之一是细胞介导的免疫反应，其中几种细胞因子似乎起主要作用。本研究的目的是检测、计数和鉴定在RAS患者外周血中分泌1型细胞因子（白细胞介素[IL]-2、IL-12、干扰素[IFN]-γ和肿瘤坏死因子[TNF]-α）和2型细胞因子（IL-4、IL-5、IL-6和IL-10）的辅助性T细胞。32例处于RAS活动期的患者（14例男性和18例女性）和40名健康个体参与了该研究。使用酶联免疫斑点测定法检测并鉴定辅助性T（T）细胞。与对照组相比，RAS患者中分泌IL-2、IL-12或IFN-γ的T细胞增加（分别为p < 0.05、p < 0.001和p < 0.001）。RAS患者和对照组中分泌TNF-α的T细胞无统计学差异（p > 0.05）。与对照组相比，RAS患者中分泌IL-10的T细胞增加（p < 0.05）。与对照组相比，RAS患者中分泌IL-4的T细胞减少（p < 0.001），RAS患者和对照组中分泌IL-5或IL-6的T细胞无统计学差异。我们的研究结果表明，分泌1型细胞因子的T细胞数量增加可能会影响针对RAS的免疫反应。这种作用是具有病因学重要性还是表观遗传现象，是一个需要回答的问题。
