Hsu Nan-Yung, Chen Chih-Yi, Hsu Chung-Ping, Lin Tze-Yi, Chou Ming-Chih, Chiou Shiow-Her, Chow Kuan-Chih
Division of Chest Surgery, China Medical University Hospital, Taichung 40227, Taiwan.
Oncol Rep. 2007 Jul;18(1):81-5.
nm23-H1, a nucleoside diphosphate kinase (NDPK), enhances drug sensitivity and has antimetastatic activity, whereas focal adhesion kinase (FAK) is closely associated with cell migration and tumour spreading. The relationship between these two proteins, however, is not well elucidated. In this study, we investigate their correlation in patients with non-small cell lung cancer (NSCLC). Expressions of nm23-H1 and FAK were examined by reverse transcription-polymerase chain reaction and immunoblotting in surgical resections. The relationship between these two genes was assessed statistically. Patients were classified into four groups according to the expression of nm23-H1 and FAK by immunohistochemistry: FAK-negative/nm23-H1-positive, FAK-negative/nm23-H1-negative, FAK-positive/nm23-H1-positive and FAK-positive/nm23-H1-negative. Although the causal correlation is still uncertain, our results showed that protein expression of nm23-H1 was inversely correlated with that of FAK. The combined analysis of nm23-H1 and FAK protein expression in the same tumour specimens revealed that patients with FAK-negative/nm23-H1-positive tumours survived the longest, 56 months, among those with nm23-H1 and FAK features (P<0.001). Our data indicate that expressions of nm23-H1 and FAK are inversely correlated. These results suggest that the status of nm23-H1 and FAK protein expression may help in predicting the aggressive behavior of NSCLC. However, further studies are warranted to clarify the impact of FAK on the function of nm23-H1 as an anti-metastatic gene.
nm23-H1是一种核苷二磷酸激酶（NDPK），可增强药物敏感性并具有抗转移活性，而粘着斑激酶（FAK）与细胞迁移和肿瘤扩散密切相关。然而，这两种蛋白质之间的关系尚未完全阐明。在本研究中，我们调查了它们在非小细胞肺癌（NSCLC）患者中的相关性。通过逆转录-聚合酶链反应和免疫印迹法检测手术切除标本中nm23-H1和FAK的表达。对这两个基因之间的关系进行统计学评估。根据免疫组织化学检测的nm23-H1和FAK表达情况，将患者分为四组：FAK阴性/nm23-H1阳性、FAK阴性/nm23-H1阴性、FAK阳性/nm23-H1阳性和FAK阳性/nm23-H1阴性。尽管因果关系仍不确定，但我们的结果表明nm23-H1的蛋白表达与FAK的蛋白表达呈负相关。对同一肿瘤标本中nm23-H1和FAK蛋白表达的联合分析显示，在具有nm23-H1和FAK特征的患者中，FAK阴性/nm23-H1阳性肿瘤患者的生存期最长，为56个月（P<0.001）。我们的数据表明nm23-H1和FAK的表达呈负相关。这些结果表明，nm23-H1和FAK蛋白表达状态可能有助于预测NSCLC的侵袭行为。然而，需要进一步研究以阐明FAK对nm23-H1作为抗转移基因功能的影响。
