Salles C A, Momen H
Department of Biochemistry and Molecular Biology, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil.
Trans R Soc Trop Med Hyg. 1991 Jul-Aug;85(4):544-7. doi: 10.1016/0035-9203(91)90251-s.
Zymovar analysis of 260 strains of Vibrio cholerae plus 3 reference strains of V. mimicus, using 13 structural loci, led to the grouping of strains in 73 zymovars (strain or group of strains sharing the same alleles). Effective separation of strains, distinction of V. cholerae strains from closely related V. mimicus and the detection of 2 vibrio strains, including one with two O1 serovars, in supposedly pure collection cultures, illustrate the potential of zymovar analysis in the identification of V. cholerae isolates. Two El Tor strains from USA, one CT+ and the other CT-, shared the same zymovar 71, while 127 typical El Tor strains belonged to zymovar 14.
使用13个结构基因座对260株霍乱弧菌以及3株模仿弧菌参考菌株进行酶谱分析，结果将这些菌株分为73个酶谱型（具有相同等位基因的菌株或菌株组）。在假定为纯培养物中，菌株的有效分离、霍乱弧菌菌株与密切相关的模仿弧菌的区分以及2株弧菌菌株（包括一株具有两种O1血清型的菌株）的检测，说明了酶谱分析在霍乱弧菌分离株鉴定中的潜力。来自美国的2株埃尔托生物型菌株，一株产霍乱毒素（CT+），另一株不产霍乱毒素（CT-），共享相同的酶谱型71，而127株典型埃尔托生物型菌株属于酶谱型14。
