[高效液相色谱法测定川芎中丁烯基苯酞的含量]
[Determination of butylidenephthalide in Ligusticum chuanxiong by HPLC].
作者信息
Wang Cheng-Yuan, Zhang Hao, Qian Zhong-Ming
机构信息
Huaxi Pharmacy College, Sichuan University, Chengdu 610041, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2007 Mar;32(6):508-10.
OBJECTIVE
To determine butylidenephthalide in Ligusticum Chuanxiong with RP-HPLC.
METHOD
The sample was extracted with methanol using sonication. The ESTD was used to quantify butylidenephthalide. HPLC separation was carried out in a Hypersil ODS columm (4.6 mm x 150 mm, 5 microm) , eluted at 1 mL x min(-1) with methanol-5% isopropyl alcohol (60: 40) at 25 degrees C. The detection wavelength was 230 nm.
RESULT
The linear range was 0.07-0.7 microg for butylidenephthalide. The average recovery was 95.3%, and RSD was 2.3% (n =6).
CONCLUSION
This method was simple and could be used to determine butylidenephthalide with satisfactory accuracy and reproducibility.
目的
采用反相高效液相色谱法（RP-HPLC）测定川芎中丁基苯酞的含量。
方法
样品用甲醇超声提取。采用外标法对丁基苯酞进行定量。在Hypersil ODS柱（4.6 mm×150 mm，5μm）上进行HPLC分离，于25℃以1 mL·min⁻¹的流速用甲醇-5%异丙醇（60∶40）洗脱，检测波长为230 nm。
结果
丁基苯酞的线性范围为0.07 - 0.7μg。平均回收率为95.3%，相对标准偏差（RSD）为2.3%（n = 6）。
结论
该方法简便，可用于测定丁基苯酞，准确性和重现性良好。
Zotero 插件