基于刃天青的96孔板微量稀释法测定生物表面活性剂的最低抑菌浓度。
Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants.
作者信息
Elshikh Mohamed, Ahmed Syed, Funston Scott, Dunlop Paul, McGaw Mark, Marchant Roger, Banat Ibrahim M
机构信息
School of Biomedical Sciences, Pharmaceutical Science and Practice Research Group, Ulster University, Cromore Road, Coleraine, County Londonderry, BT52 1SA, Northern Ireland, UK.
出版信息
Biotechnol Lett. 2016 Jun;38(6):1015-9. doi: 10.1007/s10529-016-2079-2. Epub 2016 Mar 11.
OBJECTIVES
To develop and validate a microdilution method for measuring the minimum inhibitory concentration (MIC) of biosurfactants.
RESULTS
A standardized microdilution method including resazurin dye has been developed for measuring the MIC of biosurfactants and its validity was established through the replication of tetracycline and gentamicin MIC determination with standard bacterial strains.
CONCLUSION
This new method allows the generation of accurate MIC measurements, whilst overcoming critical issues related to colour and solubility which may interfere with growth measurements for many types of biosurfactant extracts.
目的
开发并验证一种用于测量生物表面活性剂最低抑菌浓度（MIC）的微量稀释法。
结果
已开发出一种包含刃天青染料的标准化微量稀释法，用于测量生物表面活性剂的MIC，并通过使用标准菌株重复测定四环素和庆大霉素的MIC来确定其有效性。
结论
这种新方法能够进行准确的MIC测量，同时克服了与颜色和溶解性相关的关键问题，这些问题可能会干扰多种生物表面活性剂提取物的生长测量。
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