Tang Ronghua, Gao Guoqing, He Liangqiong, Han Zhuqiang, Shan Shihua, Zhong Ruichun, Zhou Cuiqiu, Jiang Jing, Li Yangrui, Zhuang Weijian
Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
J Genet Genomics. 2007 May;34(5):449-59. doi: 10.1016/S1673-8527(07)60049-6.
Peanut (Arachis hypogaea L.) is an important source crop for edible oil and protein. It is important to identify the genetic diversity of peanut genetic resources for cultivar development and evaluation of peanut accessions. Thirty-four SSR markers were used to assess the genetic variation of four sets of twenty-four accessions each from the four botanical varieties of the cultivated peanut. Among the tested accessions, ten to sixteen pairs of SSR primers showed polymorphisms. The maximum differentiation index, which was defined as the degree of genetic differentiation, was as high as 0.992 in the tested accessions. Each accession could be discriminated by a specific set of polymorphic SSR primers, and the intra-variety genetic distance was determined among accessions, with an average of 0.59 in var. fastigiata 0.46 in var. hypogaea 0.38 in var. vulgaris and 0.17 in var. hirsuta. Dendrogrames based on genetic distances were constructed for the four botanical varieties, which revealed the existence of different clusters. It was concluded that there was abundant intra-variety SSR polymorphism, and with more and more SSR markers being developed, the intrinsic genetic diversity would be detected and the development of genetic map and marker-assisted selection for cultivated peanut would be feasible.
花生（Arachis hypogaea L.）是食用油和蛋白质的重要来源作物。鉴定花生遗传资源的遗传多样性对于花生品种的培育和种质评价具有重要意义。利用34个SSR标记对栽培花生4个植物变种中每组24份种质的遗传变异进行了评估。在测试的种质中，10至16对SSR引物表现出多态性。定义为遗传分化程度的最大分化指数在测试种质中高达0.992。每个种质都可以通过一组特定的多态性SSR引物进行区分，并测定了种质间的品种内遗传距离，普通型平均为0.59，龙生型为0.46，珍珠豆型为0.38，多粒型为0.17。基于遗传距离构建了四个植物变种的聚类图，结果显示存在不同的聚类。研究得出结论，品种内SSR多态性丰富，随着越来越多SSR标记的开发，栽培花生的内在遗传多样性将被检测到，遗传图谱构建和标记辅助选择将成为可能。
