接种脊髓灰质炎病毒、柯萨奇病毒和脑心肌炎病毒的家兔中乳胶凝集抑制性IgG和IgM抗体的形成与持续存在（作者译）

[Formation and persistence of latex-agglutination-inhibiting IgG- and IgM-antibodies in rabbits inoculated with poliomyelitis, Coxsackie and encephalomyocarditis viruses (author's transl)].

作者信息

Brade H, Klein M, Schmidt W A

出版信息

Zentralbl Bakteriol Orig A. 1975 Oct;233(2):139-46.

Abstract

The formation and presistence of virus specific IgG- and IgM-antibodies produced in rabbits after a single dose of poliomyelitisvirus, coxsackievirus or encephalomyocarditisvirus were tested comparatively by latex-agglutination-inhibition test, hemagglutination-inhibition test and neutralization test. The animals were immunized either intravenously alone or with Freund's complete adjuvant. The IgM-antibodies were only detectable for a short time by the three tests. In the IgG-fraction and in the unfractionated serum the neutralizing antibodies persisted without any loss in titer during the period observed, whereas the latex-agglutination-inhibiting and hemagglutination-inhibiting antibodies showed a significant decrease. The hemagglutination-inhibiting antibodies required 23 weeks (Figure 5), the latex-agglutination-inhibiting antibodies 7 weeks (without Freund's complete adjuvant; Figure 1 and 3) or about 16 weeks (with Freund's complete adjuvant; Figure 2, 4 and 5) respectively to decline to 25% of their maximum titer.

摘要

通过乳胶凝集抑制试验、血凝抑制试验和中和试验，比较检测了家兔在单次接种脊髓灰质炎病毒、柯萨奇病毒或脑心肌炎病毒后产生的病毒特异性IgG和IgM抗体的形成及持久性。动物分别通过静脉单独免疫或与弗氏完全佐剂一起免疫。通过这三种试验，仅在短时间内可检测到IgM抗体。在所观察的时期内，IgG组分和未分级血清中的中和抗体滴度无任何损失地持续存在，而乳胶凝集抑制抗体和血凝抑制抗体则显著下降。血凝抑制抗体需要23周（图5），乳胶凝集抑制抗体分别需要7周（无弗氏完全佐剂；图1和3）或约16周（有弗氏完全佐剂；图2、4和5）下降至其最大滴度的25%。