Chichiriccò G, Picozzi P
Dipartimento di Scienze Ambientali, Università di L'Aquila, Via Vetoio, 67100 L'Aquila, Italy.
Plant Biol (Stuttg). 2007 Nov;9(6):730-5. doi: 10.1055/s-2007-965246. Epub 2007 Jun 12.
We assessed the pollen hydration, the pollen germination, and the stigma papilla penetration of CROCUS VERNUS subsp. VERNUS (Iridaceae) after 2 h fumigations with O (3), NO (2), and CO gases within humidified (90 - 100 % RH) box experiments. When the pollen and the pistil were separately fumigated, the pollen retained the capacity to emit a tube which penetrated papilla, and the stigma papillae retained the receptivity; when the pistils were first pollinated and then fumigated, the capacity of pollen to hydrate was not affected, but the germination was significantly reduced. The vulnerability to gases became evident at 0.3 ppm O (3), 0.2 ppm NO (2), and 0.5 ppm CO. The inhibition curves as a function of the gas concentrations were of an exponential type, and they saturated at 2 ppm NO (2), 25 ppm CO, and 0.5 ppm O (3), with germination percentages of 17 %, 27 %, and 60 %, respectively. Both the pollen germination and the papilla penetration were fully restored by prolonging for 60 - 90 min the incubation at 90 - 100 % RH, after the cessation of fumigations. The vulnerability of the pollen-papilla system is discussed.
在湿度为90 - 100%相对湿度的箱式实验中，我们用O₃、NO₂和CO气体熏蒸2小时后，评估了番红花（番红花属 春番红花亚种，鸢尾科）的花粉水合作用、花粉萌发以及柱头乳突穿透情况。当花粉和雌蕊分别熏蒸时，花粉保留了伸出花粉管穿透乳突的能力，柱头乳突也保留了可受性；当雌蕊先授粉然后熏蒸时，花粉水合能力未受影响，但萌发显著降低。在0.3 ppm O₃、0.2 ppm NO₂和0.5 ppm CO时，对气体的敏感性变得明显。作为气体浓度函数的抑制曲线呈指数类型，并且在2 ppm NO₂、25 ppm CO和0.5 ppm O₃时达到饱和，萌发率分别为17%、27%和60%。熏蒸停止后，在90 - 100%相对湿度下延长60 - 90分钟的孵育时间，花粉萌发和乳突穿透均完全恢复。本文讨论了花粉 - 乳突系统的敏感性。
