Gupta J, Chattopadhaya D, Bhadoria D P, Qadar Pasha M A, Gupta V K, Kumar M, Dabur R, Yadav V, Sharma G L
Institute of Genomics and Integrative Biology, University Campus, Delhi, India.
Clin Exp Immunol. 2007 Sep;149(3):463-9. doi: 10.1111/j.1365-2249.2007.03429.x. Epub 2007 Jun 12.
Chronic obstructive pulmonary disease (COPD) is an inflammatory disorder characterized by the presence of non-fully reversible airflow limitation. The study was undertaken to investigate the involvement of alpha-1-antitrypsin (alpha(1)AT) and T lymphocyte subsets in the pathogenesis of COPD. Blood samples of 50 subjects, including 25 healthy volunteers and 25 patients with COPD, were analysed. Serum trypsin inhibitory capacity (STIC) was determined by enzymatic assay. CD4(+) and CD8(+) T lymphocytes were enumerated in heparinized blood using a fluorescence activated cell sorter counter. The STIC in COPD patients was found to be decreased significantly than in controls (P < 0.01). In COPD patients with lower expression levels of alpha(1)AT, a highly significant decrease in the number of CD4(+) T lymphocytes (P < 0.0009) and CD4/CD8 ratio was observed compared with control subjects (P < 0.008). The mean +/- standard error of CD8(+) lymphocytes was found to be little different (only marginally decreased) in COPD patients compared to healthy controls; however, an alteration in the individual count of CD8(+) lymphocytes cells was observed in COPD patients. Using linear regression analysis, a negative correlation was observed between STIC and CD4(+) lymphocytes and CD8(+) lymphocytes (r = -0.40, P < 0.04; r = -0.42, P < 0.03, respectively) in COPD patients. An alteration in alpha(1)AT and T lymphocyte subsets in COPD patients suggested that interplay of these factors may be responsible for the progression of COPD.
慢性阻塞性肺疾病（COPD）是一种以存在不完全可逆性气流受限为特征的炎症性疾病。本研究旨在调查α1抗胰蛋白酶（α1AT）和T淋巴细胞亚群在COPD发病机制中的作用。对50名受试者的血样进行了分析，其中包括25名健康志愿者和25名COPD患者。通过酶法测定血清胰蛋白酶抑制能力（STIC）。使用荧光激活细胞分选仪计数器对肝素化血液中的CD4+和CD8+ T淋巴细胞进行计数。发现COPD患者的STIC明显低于对照组（P < 0.01）。在α1AT表达水平较低的COPD患者中，与对照组相比，观察到CD4+ T淋巴细胞数量显著减少（P < 0.0009），CD4/CD8比值也显著降低（P < 0.008）。与健康对照组相比，COPD患者中CD8+淋巴细胞的平均±标准误差差异不大（仅略有下降）；然而，在COPD患者中观察到CD8+淋巴细胞个体计数的改变。通过线性回归分析，在COPD患者中观察到STIC与CD4+淋巴细胞和CD8+淋巴细胞之间存在负相关（r = -0.40，P < 0.04；r = -0.42，P < 0.03）。COPD患者中α1AT和T淋巴细胞亚群的改变表明，这些因素之间的相互作用可能是COPD进展的原因。
