Fujioka Kohtaro, Geis Philip, Saito Mikako, Matsuoka Hideaki
Kobe Technical Center, Procter & Gamble Far East Inc., 17, Koyo-cho Naka 1-Chome, Higashinada-ku, Kobe, Japan.
J Ind Microbiol Biotechnol. 2007 Oct;34(10):685-8. doi: 10.1007/s10295-007-0231-7. Epub 2007 Jun 14.
An ultra-deep focusing range (UDF) fluorescent microscope system has been combined with a micromanipulation system to develop a viable cell detection-identification system applicable to microbes on environmental surfaces and products. Candida albicans yeast cells on a fabric sample surface were viably stained with a fluorescent glucose derivative, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy glucose (2-NBDG) and detected with a UDF fluorescent microscope. Visualized single-cells of C. albicans were picked in a glass microcapillary and transferred onto an agar medium. After the culture, the colony was assayed for DNA sequence to identify the isolate. This demonstrates a potential application to the study of unknown environmental microorganisms.
一种超深聚焦范围（UDF）荧光显微镜系统已与微操纵系统相结合，以开发一种适用于环境表面和产品上微生物的活细胞检测鉴定系统。织物样品表面的白色念珠菌酵母细胞用荧光葡萄糖衍生物2-[N-(7-硝基苯并-2-恶唑-1,3-二氮杂-4-基)氨基]-2-脱氧葡萄糖（2-NBDG）进行活细胞染色，并用UDF荧光显微镜进行检测。将可视化的白色念珠菌单细胞挑入玻璃微毛细管中，转移到琼脂培养基上。培养后，对菌落进行DNA序列分析以鉴定分离株。这证明了其在未知环境微生物研究中的潜在应用。
