艰难梭菌毒素检测：酶免疫测定结果与细胞毒性测定结果的比较
Detection of Clostridium difficile toxin: comparison of enzyme immunoassay results with results obtained by cytotoxicity assay.
作者信息
Musher Daniel M, Manhas Atisha, Jain Pranav, Nuila Franziska, Waqar Amna, Logan Nancy, Marino Bernard, Graviss Edward A
机构信息
Medical Service (Infectious Disease Section), Michael E. DeBakey Veterans Affairs Medical Center, Houston, Texas 77030, USA.
出版信息
J Clin Microbiol. 2007 Aug;45(8):2737-9. doi: 10.1128/JCM.00686-07. Epub 2007 Jun 13.
Abstract
Several kinds of laboratory techniques are available to detect Clostridium difficile toxin in fecal samples. Because questions have been raised about the reliability of immunoassays compared to the accepted standard, cytotoxicity assay, we studied three enzyme immunoassays (EIAs) and one rapid EIA, which demonstrated relatively good sensitivities and specificities compared to cytotoxicity assay.
摘要
有几种实验室技术可用于检测粪便样本中的艰难梭菌毒素。由于与公认的标准细胞毒性试验相比，免疫测定的可靠性受到质疑，我们研究了三种酶免疫测定法（EIA）和一种快速EIA，与细胞毒性试验相比，它们显示出相对较好的敏感性和特异性。
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