Kearney Karen, Menton John, Morgan John G
Lab 439, Department of Microbiology, University College Cork, Cork, Ireland.
Virol J. 2007 Jun 13;4:61. doi: 10.1186/1743-422X-4-61.
Noroviruses are the leading cause of infectious non-bacterial gastroenteritis in Ireland (population 4 million). Due to the number of outbreaks, its massive impact on the Irish health service and its seasonality, Norovirus has gained public notoriety as The Winter Vomiting Bug. The increase in cases in Ireland in the 2002-2003 season coincided with the emergence of two new Genogroup II genotype 4 variant clusters of Norovirus worldwide.
Little research has been done on the epidemiology or molecular biology of Norovirus strains in Ireland. In an effort to combat this discrepancy, we cloned a full length human norovirus genome as a cDNA clone (J3) which can produce full length transcripts in vitro. A polymerase mutant cDNA clone (X1), in addition to a sub genomic cDNA clone (1A) were produced for use in future work. Carlow virus (Hu/NoV/GII/Carlow/2002/Ire) genome is 7559 nts in length, excluding the 3-end poly A tail and represents the first Norovirus strain from Ireland to be sequenced.
Carlow virus is a member of the Farmington Hills variant cluster of Genogroup II genotype 4 noroviruses.
诺如病毒是爱尔兰（人口400万）传染性非细菌性肠胃炎的主要病因。由于其爆发次数、对爱尔兰医疗服务的巨大影响以及季节性，诺如病毒作为“冬季呕吐病菌”已广为人知。2002 - 2003年爱尔兰病例的增加与全球出现的两个新的诺如病毒基因II型4基因型变异簇相吻合。
爱尔兰对诺如病毒株的流行病学或分子生物学研究很少。为了克服这一差异，我们克隆了一个全长人类诺如病毒基因组作为cDNA克隆（J3），它可以在体外产生全长转录本。还制备了一个聚合酶突变体cDNA克隆（X1）以及一个亚基因组cDNA克隆（1A）用于未来的研究工作。卡洛病毒（Hu/NoV/GII/卡洛/2002/爱尔兰）基因组长度为7559个核苷酸，不包括3'端多聚A尾，是来自爱尔兰的首个被测序的诺如病毒株。
卡洛病毒是基因II型4基因型诺如病毒法明顿希尔斯变异簇的成员。
