Srinivasan Balasubramanian, Huang Xuefei
Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA.
Chirality. 2008 Mar;20(3-4):265-77. doi: 10.1002/chir.20418.
A general method is introduced to immobilize organic molecules on magnetic nanoparticles through silanization reactions and determine the maximum loading level by UV-vis spectroscopy. Loading levels of 1.5 x 10(-3) mol per gram of nanoparticle were obtained with structurally diverse compounds such as rhodamine B and glucosamine. The length of the linker did not have a significant effect on loading as comparable maximum amounts of rhodamine B were immobilized on magnetic nanoparticles regardless of the linker length. Interestingly, rhodamine B derivatives lost conjugation during synthetic manipulations due to reversible spiroisobenzofuran formation. Full regeneration of conjugation was found to be slow with rhodamine B attached on magnetic nanoparticles. The results obtained from these studies will be useful for studying surface functionalization of MNPs in general.
介绍了一种通过硅烷化反应将有机分子固定在磁性纳米颗粒上并通过紫外可见光谱法确定最大负载量的通用方法。使用结构多样的化合物如罗丹明B和葡萄糖胺，获得了每克纳米颗粒1.5×10⁻³摩尔的负载量。连接基的长度对负载量没有显著影响，因为无论连接基长度如何，相当数量的最大量罗丹明B都固定在磁性纳米颗粒上。有趣的是，由于可逆的螺异苯并呋喃形成，罗丹明B衍生物在合成操作过程中失去了共轭。发现附着在磁性纳米颗粒上的罗丹明B的共轭完全再生很慢。这些研究获得的结果总体上对于研究磁性纳米颗粒的表面功能化将是有用的。
