De Cuyper Marcel, Soenen Stefaan J H, Coenegrachts Kenneth, Beek Léon Ter
Interdisciplinary Research Centre, Katholieke Universiteit Leuven-Campus Kortrijk, B-8500 Kortrijk, Belgium.
Anal Biochem. 2007 Aug 15;367(2):266-73. doi: 10.1016/j.ab.2007.05.006. Epub 2007 May 10.
Small unilamellar phospholipid vesicles containing the phosphatidylethanolamine-diethylene triamine pentaacetic acid (PE-DTPA) conjugate as one of the building stones were constructed. The ability of these colloids to complex gadolinium(III) ions at the surface of both the inner and outer bilayer shells was verified using a colorimetric method with Arsenazo III as a dye indicator. On incubation of these functionalized vesicles with magnetoliposomes (MLs, nanometer-sized magnetite cores encapsulated in a phospholipid bilayer), PE-DTPA percolates into the ML coat. The PE-DTPA content could be fine-tuned by varying the conjugate concentration in the donor vesicles. In the experimental conditions applied, up to 500 Gd(3+) ions were immobilized per ML colloid. The resulting ML-Gd(3+) complexes might have great potential, for example, as a novel magnetic resonance imaging contrast agent.
构建了以磷脂酰乙醇胺 - 二乙烯三胺五乙酸（PE - DTPA）共轭物作为构建基石之一的小单层磷脂囊泡。使用偶氮胂III作为染料指示剂的比色法验证了这些胶体在内层和外层双层壳表面络合钆（III）离子的能力。将这些功能化囊泡与磁脂质体（MLs，包裹在磷脂双层中的纳米级磁铁矿核心）一起孵育时，PE - DTPA会渗透到ML外壳中。通过改变供体囊泡中共轭物的浓度可以微调PE - DTPA的含量。在所应用的实验条件下，每个ML胶体固定多达500个Gd(3+)离子。所得的ML - Gd(3+)复合物可能具有很大的潜力，例如作为一种新型的磁共振成像造影剂。
