de Villiers André, Górecki Tadeusz, Lynen Frédéric, Szucs Roman, Sandra Pat
Pfizer Analytical Research Centre, Ghent University, Krijgslaan 281 S4-bis, B-9000 Ghent, Belgium.
J Chromatogr A. 2007 Aug 17;1161(1-2):183-91. doi: 10.1016/j.chroma.2007.05.078. Epub 2007 May 31.
Mobile phase compensation, first reported for the charged aerosol detector (CAD), was used as a suitable method to overcome problems related to the mobile phase-dependent response of the evaporative light scattering detector (ELSD). Mobile phase compensation was effectively performed both in the flow injection- and in gradient modes. Without compensation, the response factors of the ELSD for six sulfonamide drugs differed by a factor of two when varying the mobile phase composition between 10 and 90% acetonitrile. This change could be effectively eliminated using the technique of mobile phase compensation, where a secondary pump with a reversed gradient was used to provide the detector with a constant composition of the mobile phase. For identical experimental conditions, the ELSD showed a nearly constant, albeit somewhat reduced, response with compensation. This indicates that under such conditions, the ELSD behaved as a concentration-sensitive detector. The analysis of sulfonamides drugs at 0.05% level using gradient UPLC-ELSD separation with mobile phase compensation is demonstrated.
流动相补偿最早是针对带电气溶胶检测器（CAD）报道的，它被用作一种合适的方法来克服与蒸发光散射检测器（ELSD）的流动相依赖性响应相关的问题。流动相补偿在流动注射模式和梯度模式下均能有效进行。在未进行补偿的情况下，当流动相组成在10%至90%乙腈之间变化时，ELSD对六种磺胺类药物的响应因子相差两倍。使用流动相补偿技术可以有效消除这种变化，该技术使用一个具有反向梯度的辅助泵为检测器提供恒定组成的流动相。在相同的实验条件下，ELSD在进行补偿时显示出几乎恒定的响应，尽管有所降低。这表明在这种条件下，ELSD表现为浓度敏感型检测器。本文展示了使用流动相补偿的梯度超高效液相色谱-蒸发光散射检测法在0.05%水平下对磺胺类药物的分析。
