Faghihi Shahab, Azari Fereshteh, Zhilyaev Alexander P, Szpunar Jerzy A, Vali Hojatollah, Tabrizian Maryam
Department of Biomedical Engineering, McGill University, Montreal, QC, Canada H3A 2B4.
Biomaterials. 2007 Sep;28(27):3887-95. doi: 10.1016/j.biomaterials.2007.05.010. Epub 2007 May 25.
Ultra-fine surface features are commonly used to modulate cellular activity on a variety of materials. The continuing challenge for materials in contact with bone is the development of a material with both favorable surface and bulk properties to modulate not only the cell-substrate interactions, but also to ensure the long-term stability of the implant. In a combined approach involving material sciences and cell and molecular biology, the nature and mechanism of cell-substrate interaction, in particular, the molecular machinery controlling cell response to the surface of the nanostructured titanium based material produced by the high pressure torsion (HPT) process is assessed. The degree of pre-osteoblast attachment and rate of growth, which are regulated through the activity and interaction of proteins present in the extracellular matrix and associated with cytoskeleton and focal adhesion, are notably increased on the HPT-processed titanium substrates. The improved cell activity is attributed to the nanostructured feature of these substrates consisting of ultra-fine crystals (<50 nm) and a distinct surface oxide layer which provide higher degree of surface wettability. These findings demonstrate the advantages of HPT-processed titanium over the conventional and coated titanium implants, as both mechanical properties and cellular response are improved.
超精细表面特征常用于调节各种材料上的细胞活性。与骨接触的材料面临的持续挑战是开发一种具有良好表面和整体性能的材料，不仅要调节细胞与基质的相互作用，还要确保植入物的长期稳定性。在涉及材料科学以及细胞与分子生物学的综合方法中，评估了细胞与基质相互作用的性质和机制，特别是控制细胞对通过高压扭转（HPT）工艺生产的纳米结构钛基材料表面反应的分子机制。通过细胞外基质中存在的、与细胞骨架和粘着斑相关的蛋白质的活性和相互作用来调节的前成骨细胞附着程度和生长速率，在经过HPT处理的钛基底上显著增加。细胞活性的提高归因于这些基底的纳米结构特征，其由超细晶体（<50 nm）和独特的表面氧化层组成，这提供了更高程度的表面润湿性。这些发现证明了经过HPT处理的钛相对于传统钛植入物和涂层钛植入物的优势，因为其机械性能和细胞反应都得到了改善。
