菠菜糖脂组分的抗肿瘤作用，其抑制DNA聚合酶活性。

Anti-tumor effects of the glycolipids fraction from spinach which inhibited DNA polymerase activity.

作者信息

Maeda Naoki, Kokai Yasuo, Ohtani Seiji, Sahara Hiroeki, Hada Takahiko, Ishimaru Chisato, Kuriyama Isoko, Yonezawa Yuko, Iijima Hiroshi, Yoshida Hiromi, Sato Noriyuki, Mizushina Yoshiyuki

机构信息

Laboratory of Food & Nutritional Sciences, Department of Nutritional Science, Kobe-Gakuin University, Nishi-ku, Kobe, Hyogo, Japan.

出版信息

Nutr Cancer. 2007;57(2):216-23. doi: 10.1080/01635580701277908.

DOI:10.1080/01635580701277908

PMID:17571956

Abstract

We succeeded in purifying the fraction of monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG), and sulfoquinovosyl diacylglycerol (SQDG) containing the major glycolipids from a green vegetable, spinach (Spinacia oleraceaL.). This glycolipids fraction inhibited the activities of replicative DNA polymerases (pols) such as alpha, delta, and epsilon, and mitochondrial pol gamma with IC50 values of 44.0-46.2 microg/ml, but had no influence on the activity of repair-related pol beta. The fraction also inhibited the proliferation of human cervix carcinoma (HeLa) cells with LD50 values of 57.2 microg/ml. In an in vivo anti-tumor assay on nude mice bearing solid tumors of HeLa cells, the fraction was shown to be a promising suppressor of solid tumors. Histopathological examination revealed that tumor necrosis with hemorrhage was significantly enhanced with the glycolipids fraction in vivo. The spinach glycolipids fraction might be a potent anti-tumor compound, and this fraction may be a healthy food substance with anti-tumor activity.

摘要

我们成功地从绿色蔬菜菠菜（Spinacia oleraceaL.）中纯化出了含有主要糖脂的单半乳糖基二酰基甘油（MGDG）、双半乳糖基二酰基甘油（DGDG）和磺基喹喔啉基二酰基甘油（SQDG）部分。该糖脂部分抑制了诸如α、δ和ε等复制性DNA聚合酶（pols）以及线粒体聚合酶γ的活性，IC50值为44.0 - 46.2微克/毫升，但对与修复相关的聚合酶β的活性没有影响。该部分还以57.2微克/毫升的LD50值抑制了人宫颈癌（HeLa）细胞的增殖。在对携带HeLa细胞实体瘤的裸鼠进行的体内抗肿瘤试验中，该部分被证明是一种有前景的实体瘤抑制剂。组织病理学检查显示，体内糖脂部分显著增强了伴有出血的肿瘤坏死。菠菜糖脂部分可能是一种有效的抗肿瘤化合物，并且该部分可能是一种具有抗肿瘤活性的健康食品物质。