Wilson M L, Stone B L, Hildred M V, Reves R R
Clinical Microbiology Laboratory, Denver General Hospital, Colorado 80204, USA.
J Clin Microbiol. 1995 Sep;33(9):2516-8. doi: 10.1128/jcm.33.9.2516-2518.1995.
Recovery rates from Middlebrook 7H11-selective 7H11 biplates and Lowenstein-Jensen slants (LJ) used with BACTEC 12B vials were compared for 5,399 specimens. For 578 specimens that were inoculated onto three media, 580 mycobacteria were isolated, including 277 (48%) Mycobacterium avium complex isolates, 230 (40%) Mycobacterium tuberculosis isolates, and 73 (12%) other mycobacteria. For BACTEC 12B vials, 506 (87%) cultures were positive, 45 (8%) were negative, and 29 (5%) were lost to contamination; for 7H11-7H11-selective biplates, 469 (81%) cultures were positive, 95 (16%) were negative, and 16 (3%) were lost to contamination; for LJ, 230 (40%) cultures were positive, 111 (19%) were negative, and 239 (41%) were lost to contamination. For routine cultures, use of plate media is superior to use of LJ.
对5399份标本比较了使用BACTEC 12B小瓶时Middlebrook 7H11选择性双平板和罗-琴斜面(LJ)的回收率。对于接种在三种培养基上的578份标本,分离出580株分枝杆菌,其中包括277株(48%)鸟分枝杆菌复合群分离株、230株(40%)结核分枝杆菌分离株和73株(12%)其他分枝杆菌。对于BACTEC 12B小瓶,506份(87%)培养物呈阳性,45份(8%)呈阴性,29份(5%)因污染丢失;对于7H11 - 7H11选择性双平板,469份(81%)培养物呈阳性,95份(16%)呈阴性,16份(3%)因污染丢失;对于LJ,230份(40%)培养物呈阳性,111份(19%)呈阴性,239份(41%)因污染丢失。对于常规培养,平板培养基的使用优于LJ。