在一家公共卫生分枝杆菌学实验室中，比较BACTEC 12B小瓶、Middlebrook 7H11选择性7H11双碟和罗-琴斜面培养基上分枝杆菌的回收率。
Comparison of recovery rates for mycobacteria from BACTEC 12B vials, Middlebrook 7H11-selective 7H11 biplates, and Lowenstein Jensen slants in a public health mycobacteriology laboratory.
作者信息
Wilson M L, Stone B L, Hildred M V, Reves R R
机构信息
Clinical Microbiology Laboratory, Denver General Hospital, Colorado 80204, USA.
出版信息
J Clin Microbiol. 1995 Sep;33(9):2516-8. doi: 10.1128/jcm.33.9.2516-2518.1995.
Abstract
Recovery rates from Middlebrook 7H11-selective 7H11 biplates and Lowenstein-Jensen slants (LJ) used with BACTEC 12B vials were compared for 5,399 specimens. For 578 specimens that were inoculated onto three media, 580 mycobacteria were isolated, including 277 (48%) Mycobacterium avium complex isolates, 230 (40%) Mycobacterium tuberculosis isolates, and 73 (12%) other mycobacteria. For BACTEC 12B vials, 506 (87%) cultures were positive, 45 (8%) were negative, and 29 (5%) were lost to contamination; for 7H11-7H11-selective biplates, 469 (81%) cultures were positive, 95 (16%) were negative, and 16 (3%) were lost to contamination; for LJ, 230 (40%) cultures were positive, 111 (19%) were negative, and 239 (41%) were lost to contamination. For routine cultures, use of plate media is superior to use of LJ.
摘要
对5399份标本比较了使用BACTEC 12B小瓶时Middlebrook 7H11选择性双平板和罗-琴斜面（LJ）的回收率。对于接种在三种培养基上的578份标本，分离出580株分枝杆菌，其中包括277株（48%）鸟分枝杆菌复合群分离株、230株（40%）结核分枝杆菌分离株和73株（12%）其他分枝杆菌。对于BACTEC 12B小瓶，506份（87%）培养物呈阳性，45份（8%）呈阴性，29份（5%）因污染丢失；对于7H11 - 7H11选择性双平板，469份（81%）培养物呈阳性，95份（16%）呈阴性，16份（3%）因污染丢失；对于LJ，230份（40%）培养物呈阳性，111份（19%）呈阴性，239份（41%）因污染丢失。对于常规培养，平板培养基的使用优于LJ。
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