Cottell E, Lennon B, McMorrow J, Barry-Kinsella C, Harrison R F
Royal College of Surgeons in Ireland, Academic Department of Obstetrics and Gynaecology, Rotunda Hospital, Dublin.
Fertil Steril. 1997 Jan;67(1):98-103. doi: 10.1016/s0015-0282(97)81863-8.
To identify and determine the prevalence of microorganisms in preprocessed and postprocessed semen in an IVF-ET program.
Prospective study.
University Teaching Hospital.
PATIENT(S): Seventy-four men undergoing preprogram evaluation, each producing two semen samples.
INTERVENTION(S): Semen processing with a wash and swim-up technique in a penicillin- and streptomycin-rich medium.
MAIN OUTCOME MEASURE(S): The identity and prevalence of seminal microorganisms before and after processing.
RESULT(S): Sixty-three percent of individual unprocessed semen samples grew microorganisms, the majority of which were nonpathogenic. Thirty-three men (44.6%) had microbes identified in repeat samples, four had identical organisms each time. Twenty (27%) had positive cultures in one sample, negative in the other. Twenty-one (28.4%) had consistently sterile semen. After seminal processing, the recovery rate for microbes was 5%. Microbial presence after processing did not correlate with either the sperm swim-up concentration or the initial microbial concentration.
CONCLUSION(S): Bacteriospermia is common. The microorganisms found rarely are replicated and most likely represent contamination. Wash and swim-up semen preparation in an antibiotic rich culture medium effectively eliminates 95% of organisms.
确定体外受精 - 胚胎移植（IVF - ET）项目中预处理前后精液里微生物的种类及流行情况。
前瞻性研究。
大学教学医院。
74名接受项目前评估的男性，每人提供两份精液样本。
在富含青霉素和链霉素的培养基中采用洗涤和上游法处理精液。
处理前后精液中微生物的种类及流行情况。
63%的未处理个体精液样本培养出微生物，其中大多数为非致病性微生物。33名男性（44.6%）的重复样本中鉴定出微生物，4名每次鉴定出的微生物相同。20名（27%）在一个样本中培养结果为阳性，另一个样本为阴性。21名（28.4%）精液一直无菌。精液处理后，微生物的回收率为5%。处理后微生物的存在与精子上游浓度或初始微生物浓度均无相关性。
菌精症很常见。发现的微生物很少能被复制，很可能是污染所致。在富含抗生素的培养基中进行洗涤和上游法精液制备可有效清除95%的微生物。
