Guillet-Rosso F, Fari A, Taylor S, Forman R, Belaisch-Allart J, Testart J, Frydman R
Br J Obstet Gynaecol. 1987 Jun;94(6):543-7. doi: 10.1111/j.1471-0528.1987.tb03148.x.
Semen specimens from 519 men were cultured for organisms 2 weeks before oocyte recovery for in-vitro fertilization (IVF). The cultures from 72 men contained more than 5000 bacteria/ml and the IVF attempt was postponed. The IVF outcome was assessed in 382 couples in relation to the semen culture result. The pregnancy rate per cycle was significantly reduced when the semen culture contained organisms compared with axenic semen (P less than 0.05). This was independent of the cleavage rate of oocytes and the number of embryos transferred. Although mycoplasma was frequently cultured, chlamydial infection was very rare and no viruses were isolated in this study.
在进行体外受精（IVF）的卵母细胞回收前2周，对519名男性的精液标本进行了微生物培养。72名男性的培养物中细菌含量超过5000个/毫升，因此IVF尝试被推迟。根据精液培养结果，对382对夫妇的IVF结局进行了评估。与无菌精液相比，当精液培养中有微生物时，每个周期的妊娠率显著降低（P<0.05）。这与卵母细胞的分裂率和移植的胚胎数量无关。尽管支原体培养很常见，但衣原体感染非常罕见，且本研究中未分离出病毒。
