Chen Guang, Lu Fu-er, Jin Dan, Xu Li-jun, Wang Kai-fu
Institute of Integrative Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Chin J Integr Med. 2007 Mar;13(1):41-5. doi: 10.1007/s11655-007-0041-9.
To investigate the effects of Huanglian Jiedu Decoction (HLJDD) on glucose transporter 4 (GLUT4) protein expressions in insulin-resistant murine target tissues.
The experimental male Wistar rats were established into insulin resistant models by injecting streptozotocin (STZ 30 mg/kg) via caudal vein and feeding them with high fat high caloric diet, and randomly divided into the model group, the aspirin group and the HLJDD group. Besides, a normal group was set up for control. Changes of body weight (BW), levels of serum fasting blood glucose (FBG), serum fasting insulin (FINS) and oral glucose tolerance test (OGTT) were routinely determined. The expression of GLUT4 protein in adipose and skeletal muscle tissues before and after insulin stimulation was determined with Western blot.
In the HLJDD group after treatment, BW and FBG got decreased, OGTT improved, and the expression and translocation of GLUT4 protein elevated obviously, either before or after insulin stimulation, as compared with those in the model group, showing significant differences respectively.
The mechanism of improving insulin resistance by HLJDD is probably associated with its effect in elevating GLUT4 protein expression and translocation in adipose and skeletal muscle tissues of insulin resistant rats.
探讨黄连解毒汤（HLJDD）对胰岛素抵抗小鼠靶组织中葡萄糖转运蛋白4（GLUT4）蛋白表达的影响。
将实验雄性Wistar大鼠经尾静脉注射链脲佐菌素（STZ 30 mg/kg）并给予高脂高糖饮食，建立胰岛素抵抗模型，随机分为模型组、阿司匹林组和HLJDD组。此外，设正常组作为对照。常规测定体重（BW）、血清空腹血糖（FBG）水平、血清空腹胰岛素（FINS）水平及口服葡萄糖耐量试验（OGTT）。采用蛋白质免疫印迹法检测胰岛素刺激前后脂肪和骨骼肌组织中GLUT4蛋白的表达。
治疗后HLJDD组体重和空腹血糖降低，口服葡萄糖耐量试验改善，胰岛素刺激前后GLUT4蛋白的表达及转位均明显升高，与模型组比较，差异均有统计学意义。
黄连解毒汤改善胰岛素抵抗的机制可能与其升高胰岛素抵抗大鼠脂肪和骨骼肌组织中GLUT4蛋白表达及转位有关。
