Zhu Qing-Feng, Zhang Fa-Bao, Wang Ke-Ming, Zhou Yi-Ping
Department of Life Sciences, Anqing Teachers College, Anqing 246011, China.
Zhen Ci Yan Jiu. 2007 Feb;32(1):20-3.
To probe into the relationship between "Xinshu" (BL 15)-"Jueyinshu" (BL 14) of the Bladder Channel and the heart and the underlying mechanism of electroacupuncture (EA) in resisting acute myocardial ischemia (AMI) in rats.
A total of 24 male Wistar rats were randomly divided into control, model and EA groups with 8 cases in each group. AMI model was duplicated by intraperitoneal injection of 2% isoproterenol (ISO, 100 mg/kg). EA (pulse duration 300 micros, frequency 2-20 Hz, output voltage 3. 5-5 V) was applied to BL14 and BL15 on the left side for 30 min, twice in a day (with an interval of 12 h). After decapitation, blood samples, myocardial tissue and spinal cord (T1-T6) were collected respectively for detecting serum creatine kinase (CK, with chromatometry) and calcitonin gene-related peptide (CGRP) contents in the plasma, myocardium and the spinal cord (with radioimmunoassay).
In comparison with control group, serum CK of both model group and EA group increased significantly (P < 0. 01), and plasma CGRP content of model group lowered lightly; while compared with model group, CK content of EA group decreased obviously (P < 0.05) and plasma CGRP of EA group increased evidently (P < 0.05). No significant differences were found among 3 groups in myocardial CGRP contents and between control group and model group in spinal CGRP contents (P > 0.05).
EA of "Xinshu" (BL 15)-"Jueyinshu" (BL 14) may suppress isoproterenol-injection induced myocardial injury and plasma CGRP possibly takes part in the protective effect of EA in resisting myocardial ischemia injury.
探讨膀胱经“心俞”（BL 15）-“厥阴俞”（BL 14）与心脏的关系及电针抗大鼠急性心肌缺血（AMI）的作用机制。
将24只雄性Wistar大鼠随机分为对照组、模型组和电针组，每组8只。腹腔注射2%异丙肾上腺素（ISO，100 mg/kg）复制AMI模型。电针（脉冲宽度300 μs，频率2 - 20 Hz，输出电压3.5 - 5 V）左侧BL14、BL15穴30 min，每日2次（间隔12 h）。断头后分别采集血样、心肌组织和脊髓（T1 - T6），采用比色法检测血清肌酸激酶（CK），采用放射免疫法检测血浆、心肌和脊髓中降钙素基因相关肽（CGRP）含量。
与对照组比较，模型组和电针组血清CK均显著升高（P < 0.01），模型组血浆CGRP含量轻度降低；与模型组比较，电针组CK含量明显降低（P < 0.05），电针组血浆CGRP明显升高（P < 0.05）。3组心肌CGRP含量及对照组与模型组脊髓CGRP含量比较差异无统计学意义（P > 0.05）。
电针“心俞”（BL 15）-“厥阴俞”（BL 14）可抑制异丙肾上腺素所致心肌损伤，血浆CGRP可能参与电针抗心肌缺血损伤的保护作用。
