[电针“原络配穴”对急性心肌缺血家兔心功能及心肌酶的影响]

[Effects of electroacupuncture of "source-" plus "collateral-" acupoints on the cardiac function and myocardial enzymes in acute myocardial ischemia rabbits].

作者信息

Hu Ling, He Lu, Cai Rong-Lin, Long Di-He, Wu Zi-Jian

机构信息

Research Institute of Acu-Moxibustion and Meridians, Anhui College of Chinese Medicine, Anhui Key Laboratory of Foundation and Technology of Acu-Moxibustion Cultivating Base, Hefei 230038, China.

出版信息

Zhen Ci Yan Jiu. 2010 Oct;35(5):363-7.

PMID:21235065

Abstract

OBJECTIVE

To observe the effects of electroacupuncture (EA) of "Source-" + "Collateral-" acupoints ["Shenmen" (HT 7) + "Zhizheng"(SI 7)] and single acupoints "Neiguan"(PC 6) and HT 7 on cardiac function and myocardial enzymes in acute myocardial ischemia (AMI) rabbits.

METHODS

Sixty Chinchilla rabbits were randomized into normal control, model, Shenmen (HT 7), Neiguan (PC 6), HT 7 + SI 7 groups, with 8 rabbits in each. AMI model was duplicated by occlusion of the anterior descending branch of left coronary artery under anesthesia. EA (2 Hz,1 mA) was applied to acupoints HT 7, PC 6,and HT 7 + SI 7 for 10 min. The maximum ascending rate (+ dp/dt max) and maximum descending rate (-dp/dt max) of the left ventricular pressure (LVP) were recorded by using PowerLab System. Serum lactate dehydrogenase (LDH) and creatine kinase MB (CK-MB) contents were detected with chromatometry.

RESULTS

Serum LDH and OK-MB contents increased significantly in model group than in normal control group (P < 0.01). Compared with model group, serum LDH and CK-MB contents decreased remarkably in PC 6, HT 7 and HT 7 + SI 7 groups (P < 0.01, P < 0.05), and the effects of HT 7 + SI 7 group were significantly superior to those of PC 6 and HT 7 groups in lowering serum LDH and CK-MB levels (P < 0.01, P < 0.05). Compared with the normal control group, + dp/dt max lowered significantly and -dp/dt max increased markedly in the model group (P < 0.01), while compared with the model group, + dp/dt max increased obviously and -dp/dt max decreased significantly in the three EA groups (P < 0.01, P < 0.05). The effects of HT 7 + SI 7 group were markedly superior to those of HT 7 and PC 6 in raising + dp/dt max and lowering -dp/dt max (P < 0.05, P < 0.01), and those effects of PC 6 group were evidently better than those of HT 7 group (P < 0.05, P < 0.01).

CONCLUSION

EA of "Source-" + "Collateral-" aoupoints (HT 7 + SI 7), HT 7 and PC 6 has a positive effect in improving changes of cardiac function and in lowering serum LDH and CK-MB contents in AMI rabbits, and the effect of EA of HT 7 + SI 7 is superior to that of EA of single acupoint.

摘要

目的

观察电针“原络”配穴[“神门”（HT 7）+“支正”（SI 7）]、单穴“内关”（PC 6）及“神门”（HT 7）对急性心肌缺血（AMI）家兔心功能及心肌酶的影响。

方法

将60只青紫蓝兔随机分为正常对照组、模型组、神门（HT 7）组、内关（PC 6）组、HT 7 + SI 7组，每组8只。麻醉下结扎左冠状动脉前降支复制AMI模型。分别对HT 7、PC 6、HT 7 + SI 7穴进行电针（2 Hz，1 mA）刺激10分钟。采用PowerLab系统记录左心室压力（LVP）的最大上升速率（+ dp/dt max）和最大下降速率（-dp/dt max）。用比色法检测血清乳酸脱氢酶（LDH）和肌酸激酶同工酶MB（CK-MB）含量。

结果

模型组血清LDH和CK-MB含量较正常对照组显著升高（P < 0.01）。与模型组比较，PC 6组、HT 7组和HT 7 + SI 7组血清LDH和CK-MB含量显著降低（P < 0.01，P < 0.05），且HT 7 + SI 7组降低血清LDH和CK-MB水平的作用显著优于PC 6组和HT 7组（P < 0.01，P < 0.05）。与正常对照组比较，模型组+ dp/dt max显著降低，-dp/dt max显著升高（P < 0.01）；与模型组比较，3个电针组+ dp/dt max明显升高，-dp/dt max显著降低（P < 0.01，P < 0.05）。HT 7 + SI 7组在提高+ dp/dt max和降低-dp/dt max方面的作用显著优于HT 7组和PC 6组（P < 0.05，P < 0.01），PC 6组的作用明显优于HT 7组（P < 0.05，P < 0.01）。