Mazyad Said A M, Hafez Adel Omar
Research and Training Center on Vectors of Diseases, Ain Shams University, Cairo 11566, Egypt.
J Egypt Soc Parasitol. 2007 Apr;37(1):135-42.
Antibodies against Coxiella burnetii were estimated among sheep, goats and camels (190), their owners (150 patients with pyrexia of unknown origin) and 30 normal individuals in North Sinai over the 2006 by indirect immounofluorescence assay. Nested polymerase chain reaction was used to detect Com-1 gene (genetic target of C. burnetii) encoding a 27-kDa outer membrane protein in the samples. C. burnetii IFA antibodies (IgM & IgG) in patients were 8 (5.3%) and a healthy control (3.3%). The overall was 9 of 180 (5.0%). C. burnetii IgM were detected in 3/150 (2%) patients with positive genome, while IgG were detected in 5/150 patients, only the three who had IgM and IgG had positive genome suffered high fever. C. burnetii antibodies were detected in 20 (22.5%), 12 (16.8%) & 4 (13.3%) of sheep, goats, camels, which total 36/190 (18.9%). The positive genome of these IFA positive animals was 10 (50.0%), 4 (33.3%) & zero (0.0%) respectively. On the other hand, Rhipicephalus sanguineus (dog tick) and Dermacentor andersoni (wood tick) were identified on some Q fever infected animals. The results were discussed.
2006年期间，通过间接免疫荧光测定法，对北西奈地区的绵羊、山羊和骆驼（190只）、它们的主人（150名不明原因发热患者）以及30名正常个体体内的抗伯氏考克斯体抗体进行了评估。采用巢式聚合酶链反应检测样本中编码27 kDa外膜蛋白的Com-1基因（伯氏考克斯体的基因靶点）。患者体内的伯氏考克斯体免疫荧光抗体（IgM和IgG）分别有8例（5.3%）和1例健康对照（3.3%）。180例中的总体阳性率为9例（5.0%）。在3/150（2%）基因组阳性的患者中检测到伯氏考克斯体IgM，而在5/150例患者中检测到IgG，只有同时具有IgM和IgG且基因组阳性的3例患者出现高热。在绵羊、山羊、骆驼中分别有20例（22.5%）、12例（16.8%）和4例（13.3%）检测到伯氏考克斯体抗体，总计36/190（18.9%）。这些免疫荧光检测阳性动物的基因组阳性率分别为10例（50.0%）、4例（33.3%）和0例（0.0%）。另一方面，在一些感染Q热的动物身上发现了血红扇头蜱（狗蜱）和安氏革蜱（木蜱）。对结果进行了讨论。
