[环维黄杨星-D对大鼠脑缺血再灌注损伤的影响]
[Effect of cyclovirobuxinum-D on cerebral ischemia-reperfusion injury in rats].
作者信息
Yu Hao, Liu Han-Zhen, Zhou Guo-Liang
机构信息
Anhui Science and Technology University, Bengbu 233100, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2007 Apr;32(7):623-6.
OBJECTIVE
To observe the effect of cyclovirobuxinum-D (CVB-D) on cerebral ischemia-reperfusion injury in rats and explore its mechanisms.
METHOD
One hundred and twenty rats were randomly divided into three CVB-D groups (2, 1, 0.5 mg x kg(-1)), Nimodipine group (2 mg x kg(-1)), model group and sham operated group, 20 rats each group. Rat cerebral ischemia-reperfusion injury model was induced by middle cerebral artery occlusion, the nerve injury symptoms was evaluated, the level of SOD and MDA in brain tissue were determined, the concentration of intracellar Ca2+ of brain was measured, and the pathological change of brain was also observed.
RESULT
CVB-D could improve the nerve injury symptoms, reduce the infarction area of brain, the concentration of intracellar Ca2+ and the level of MDA, increase the activity of SOD, and decrease the pathological change of brain.
CONCLUSION
CVB-D has protective effect on cerebral ischemia-reperfusion injury in rats.
目的
观察环维黄杨星-D（CVB-D）对大鼠脑缺血再灌注损伤的影响并探讨其作用机制。
方法
将120只大鼠随机分为CVB-D高、中、低剂量组（2、1、0.5mg·kg⁻¹）、尼莫地平组（2mg·kg⁻¹）、模型组和假手术组，每组20只。采用大脑中动脉闭塞法制备大鼠脑缺血再灌注损伤模型，评价神经损伤症状，测定脑组织中超氧化物歧化酶（SOD）和丙二醛（MDA）水平，检测脑细胞内Ca²⁺浓度，并观察脑组织病理变化。
结果
CVB-D可改善神经损伤症状，缩小脑梗死面积，降低脑细胞内Ca²⁺浓度和MDA水平，提高SOD活性，减轻脑组织病理改变。
结论
CVB-D对大鼠脑缺血再灌注损伤具有保护作用。
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