川芎嗪-葛根素对大鼠大脑中动脉短暂闭塞所致局灶性脑缺血模型的保护作用

Protective effect of chuanxiongzine-puerarin in a rat model of transient middle cerebral artery occlusion-induced focal cerebral ischemia.

作者信息

Wan Haitong, Zhu Huiyuan, Tian Mei, Hu Xiaolei, Yang Jiehong, Zhao Chunlei, Zhang Hong

机构信息

Institute of Cardio-Cerbrovascular Disease, Zhejiang Chinese Medical University, China.

出版信息

Nucl Med Commun. 2008 Dec;29(12):1113-22. doi: 10.1097/MNM.0b013e3283108995.

DOI:10.1097/MNM.0b013e3283108995

PMID:18987534

Abstract

AIM

The aim of this study was to investigate the protective effect of chuanxiongzine-puerarin on cerebral ischemia-reperfusion damage in a rat model.

METHODS

Rats were randomized into the following three groups: control group (sham operation group), model group (cerebral ischemia-reperfusion damage group), and treatment group (puerarin group, chuanxiongzine-puerarin group, and nimodipine group). The effect of neurological deficit, infarct volume and levels of superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO), synaptophysin, and interleukin-1 was assessed. The therapeutic effect was also evaluated micro-positive emission tomography with [F]fluoro-2-deoxy-D-glucose.

RESULTS

After cerebral ischemia-reperfusion damage, the neurologic deficit score and the infarct volume percentage in treatment group were significantly lower than in the model group (P<0.01). Micro-positive emission tomography imaging showed that glucose metabolism in the right temporal lobe and the right frontal lobe were significantly decreased in the model group in comparison with the sham operation group (P<0.01). After treatment, glucose metabolism increased significantly in the puerarin group and the chuanxiongzine-puerarin group, compared with the model group (P<0.05 and <0.01, respectively). The expression of interleukin-1beta-mRNA was significantly higher in the model group than in the sham operation group (P<0.01). After treatment, however, it was significantly decreased in the puerarin group (P<0.05) and the chuanxiongzine-puerarin group (P<0.01) compared with the model group. SOD activity in the model group (P<0.01) was lower than that in the sham operation group, whereas MDA and NO content in the model group was more than that in the sham operation group (P<0.01). In the chuanxiongzine-puerarin and puerarin treatment groups, SOD activity showed higher level compared with the model group (P<0.01); however, MDA and NO content was decreased in the treatment group (P<0.01). Synaptophysin level was significantly lower in the model group compared with the sham operation group (P<0.01), whereas the chuanxiongzine-puerarin and puerarin groups showed significantly higher values than the model group (P<0.05).

CONCLUSION

This study showed that chuanxiongzine-puerarin played an important protective role against cerebral ischemic reperfusion damage in a rat model.

摘要

目的

本研究旨在探讨川芎嗪 - 葛根素对大鼠脑缺血再灌注损伤的保护作用。

方法

将大鼠随机分为以下三组：对照组（假手术组）、模型组（脑缺血再灌注损伤组）和治疗组（葛根素组、川芎嗪 - 葛根素组和尼莫地平组）。评估神经功能缺损、梗死体积以及超氧化物歧化酶（SOD）、丙二醛（MDA）、一氧化氮（NO）、突触素和白细胞介素 -1水平的影响。还采用[F]氟代 -2-脱氧 -D-葡萄糖进行微正电子发射断层扫描评估治疗效果。

结果

脑缺血再灌注损伤后，治疗组的神经功能缺损评分和梗死体积百分比显著低于模型组（P<0.01）。微正电子发射断层扫描成像显示，与假手术组相比，模型组右侧颞叶和右侧额叶的葡萄糖代谢显著降低（P<0.01）。治疗后，与模型组相比，葛根素组和川芎嗪 - 葛根素组的葡萄糖代谢显著增加（分别为P<0.05和<0.01）。模型组白细胞介素 -1β -mRNA的表达显著高于假手术组（P<0.01）。然而，治疗后，与模型组相比，葛根素组（P<0.05）和川芎嗪 - 葛根素组（P<0.01）的该表达显著降低。模型组的SOD活性低于假手术组（P<0.01），而模型组的MDA和NO含量高于假手术组（P<0.01）。在川芎嗪 - 葛根素和葛根素治疗组中，SOD活性与模型组相比显示较高水平（P<0.01）；然而，治疗组的MDA和NO含量降低（P<0.01）。模型组的突触素水平显著低于假手术组（P<0.01），而川芎嗪 - 葛根素组和葛根素组的值显著高于模型组（P<0.05）。