Landgraf R, Neumann I, Pittman Q J
Department of Cell Biology and Regulation, University of Leipzig, FRG.
Neuroendocrinology. 1991 Oct;54(4):378-83. doi: 10.1159/000125917.
The push-pull perfusion technique, in conjunction with specific radioimmunoassays, was used to monitor the release of both arginine vasopressin (AVP) and oxytocin (OXT) within distinct limbic brain areas of conscious female rats. In pregnant rats near term, the release of AVP was greater than that of virgin rats in both the ventral (p less than 0.001) and mediolateral (p less than 0.001) septal areas; similarly, release of OXT increased in the ventral septal area (p less than 0.01) at this time. In contrast, no changes in the levels of either peptide occurred in the dorsal hippocampus. In parturient rats, AVP release tended to decrease in the septal areas but increased fivefold in the dorsal hippocampus (p less than 0.001) compared to pregnant animals. In contrast, OXT levels assayed in the same perfusates did not differ from those observed in pregnant animals. Plasma levels of AVP in pregnant rats (p less than 0.05) and of OXT in parturient animals (p less than 0.01) were found to be increased over levels in virgin rats. The regionally different and peptide-specific changes in release pattern of AVP and OXT in virgin, pregnant and parturient rats may be of physiological significance in antipyresis and behaviors accompanying parturition.
推挽式灌注技术结合特定的放射免疫测定法，用于监测清醒雌性大鼠不同边缘脑区中精氨酸加压素（AVP）和催产素（OXT）的释放。在近足月的妊娠大鼠中，腹侧（p<0.001）和中外侧（p<0.001）隔区中AVP的释放量均高于未孕大鼠；同样，此时腹侧隔区中OXT的释放量增加（p<0.01）。相比之下，背侧海马体中这两种肽的水平均未发生变化。在分娩大鼠中，隔区中AVP的释放量趋于下降，但与妊娠动物相比，背侧海马体中的释放量增加了五倍（p<0.001）。相比之下，在相同灌注液中检测到的OXT水平与妊娠动物中观察到的水平没有差异。发现妊娠大鼠的血浆AVP水平（p<0.05）和分娩动物的血浆OXT水平（p<0.01）高于未孕大鼠。未孕、妊娠和分娩大鼠中AVP和OXT释放模式的区域差异和肽特异性变化可能在解热和分娩相关行为中具有生理意义。
