Gaetje Regine, Holtrich Uwe, Karn Thomas, Cikrit Eva, Engels Knut, Rody Achim, Kaufmann Manfred
Department of Obstetrics and Gynecology, Johann Wolfgang Goethe-University, Frankfurt, Germany.
Fertil Steril. 2007 Dec;88(6):1534-40. doi: 10.1016/j.fertnstert.2007.01.128. Epub 2007 Jun 27.
To characterize the expression of WNT7A in human eutopic and ectopic endometrium.
Experimental study using real-time polymerase chain reaction, laser microdissection, in situ hybridization, and immunofluorescence.
University-based laboratory.
PATIENT(S): Patients with and without endometriosis undergoing surgery for benign indications.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Relative expression values compared with housekeeping genes using real-time polymerase chain reaction. Detection of positive cells by immunofluorescence and in situ hybridization.
RESULT(S): In endometriosis, statistically significant higher WNT7A mRNA expression was observed compared with eutopic endometrium. Expression of WNT7A was found in the luminal and glandular epithelial cells as well as stroma cells in endometrium and endometriosis by immunofluorescence, in situ hybridization, and polymerase chain reaction of laser microdissected tissue.
CONCLUSION(S): The results of the present study suggest that WNT7A plays a role in the pathophysiology of endometriosis.
表征WNT7A在人在位及异位子宫内膜中的表达情况。
采用实时聚合酶链反应、激光显微切割、原位杂交和免疫荧光的实验研究。
大学实验室。
因良性指征接受手术的有或无子宫内膜异位症的患者。
无。
使用实时聚合酶链反应与管家基因比较相对表达值。通过免疫荧光和原位杂交检测阳性细胞。
在子宫内膜异位症中，与在位子宫内膜相比，观察到WNT7A mRNA表达有统计学意义的显著升高。通过免疫荧光、原位杂交以及激光显微切割组织的聚合酶链反应发现，在位子宫内膜和子宫内膜异位症的腔上皮细胞、腺上皮细胞以及基质细胞中均有WNT7A表达。
本研究结果表明WNT7A在子宫内膜异位症的病理生理学中发挥作用。
