Li Yanzi, Rethwisch David G
Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, Iowa 52242, USA.
Biotechnol Bioeng. 2002 Jul 5;79(1):15-22. doi: 10.1002/bit.10272.
The successful scale-up of the enzymatic synthesis of alpha-methyl glucoside acrylate from laboratory-scale (milliliter) to pilot-scale (liter) was examined. Specifically, Candida antarctica lipase B (Novozym 435) was used as a biocatalyst to produce alpha-methyl glucoside acrylate via the transesterification of alpha-methyl glucoside (MG) with vinyl acrylate (VA) using acetone as a solvent. This is a pseudo-solid-phase synthesis; only a fraction of the alpha-methyl glucoside and the product are soluble in acetone. Molecular sieves were used to remove traces of water in the reaction medium and to increase enzyme stability by removing the acetaldehyde by-product. A general method was also developed to purify and recover the monoacrylate product from unreacted sugar and undesired diester by a simple crystallization and precipitation process.
研究了从实验室规模（毫升）到中试规模（升）成功扩大α-甲基葡糖苷丙烯酸酯酶促合成的规模。具体而言，使用南极假丝酵母脂肪酶B（诺维信435）作为生物催化剂，以丙酮为溶剂，通过α-甲基葡糖苷（MG）与丙烯酸乙烯酯（VA）的酯交换反应制备α-甲基葡糖苷丙烯酸酯。这是一种准固相合成；只有一小部分α-甲基葡糖苷和产物可溶于丙酮。使用分子筛去除反应介质中的痕量水分，并通过去除乙醛副产物来提高酶的稳定性。还开发了一种通用方法，通过简单的结晶和沉淀过程从未反应的糖和不需要的二酯中纯化和回收单丙烯酸酯产物。
