Herzog Grégoire, Arrigan Damien W M
Tyndall National Institute, Lee Maltings, University College, Cork, Ireland.
Analyst. 2007 Jul;132(7):615-32. doi: 10.1039/b701472d. Epub 2007 May 16.
Electrochemical methods for the detection of amino acids, peptides, and proteins in a variety of media are reviewed. Label-free strategies in which the detection is based on the inherent electrochemical properties of the analyte are discussed. Various processes such as direct or mediated (in solution or immobilised) redox processes and interfacial ion transfers have been employed for the electrochemical detection and determination of the target analytes. The various methods covered encompass voltammetry at uncoated and modified electrodes and at immiscible liquid-liquid interfaces, potentiometry at polymer membrane electrodes and electrochemical impedance spectroscopy. The determination of the target analytes in complex biological matrices is discussed. The various approaches highlighted here illustrate the rich capabilities of electrochemical methods as simple, low-cost, sensitive tools for the determination of these important biological analytes at trace and ultra-trace levels.
本文综述了用于检测多种介质中氨基酸、肽和蛋白质的电化学方法。讨论了基于分析物固有电化学性质的无标记策略。各种过程，如直接或介导（溶液中或固定化）的氧化还原过程和界面离子转移，已被用于目标分析物的电化学检测和测定。涵盖的各种方法包括在未涂层和修饰电极以及不混溶液-液界面处的伏安法、聚合物膜电极处的电位分析法和电化学阻抗谱。还讨论了在复杂生物基质中目标分析物的测定。这里强调的各种方法表明，电化学方法作为简单、低成本、灵敏的工具，在痕量和超痕量水平测定这些重要生物分析物方面具有丰富的能力。
