McFadden B A, Purohit S
J Bacteriol. 1977 Jul;131(1):136-44. doi: 10.1128/jb.131.1.136-144.1977.
Enzymes catalyzing steps from ethanol to acetyl-coenzyme A, from malate to pyruvate, and from pyruvate to glucose 6-phosphate were identified in ethanol-grown Pseudomonas indigofera. Enzymes catalyzing the catabolism of glucose to pyruvate via the Entner-Doudoroff pathway were identified in glucose-grown cells. Phosphofructokinase could not be detected in Pseudomonas indigofera. Itaconate, a potent inhibitor of isocitrate lyase, abolished growth of P. indigofera on ethanol at concentrations that had little effect upon growth on glucose. The date obtained through enzyme analyses and studies of itaconate inhibition with both extracts and toluene-treated cells suggest that itaconate selectively inhibits and reduces the specific activity of isocitrate lyase.
在以乙醇为生长底物的产靛假单胞菌中，鉴定出了催化从乙醇到乙酰辅酶A、从苹果酸到丙酮酸以及从丙酮酸到6-磷酸葡萄糖步骤的酶。在以葡萄糖为生长底物的细胞中，鉴定出了通过恩特纳-杜德洛夫途径催化葡萄糖分解代谢为丙酮酸的酶。在产靛假单胞菌中未检测到磷酸果糖激酶。衣康酸是异柠檬酸裂合酶的有效抑制剂，在对葡萄糖生长影响很小的浓度下，衣康酸会抑制产靛假单胞菌在乙醇上的生长。通过酶分析以及对提取物和经甲苯处理的细胞进行衣康酸抑制研究获得的数据表明，衣康酸选择性抑制并降低异柠檬酸裂合酶的比活性。
