Greenstein A, Aravot D, Braf Z, Lelcuk S
Department of Urology, Tel-Aviv Sourasky Medical Center, Israel.
Urol Res. 1991;19(6):393-5. doi: 10.1007/BF00310157.
The present study was designed to determine whether the administration of superoxide dismutase (SOD) can alleviate ischemic kidney damage and whether there is a relationship between oxygen free radicals and thromboxane (Tx). In 17 dogs, the right kidney was removed and the vascular pedicle of the left kidney was clamped for 75 min. Prior to reperfusion, the ischemic kidney was rinsed with 5 mg SOD and an additional 20 mg SOD was infused systemically. Blood samples were drawn from the renal vein before ischemia and after reperfusion to determine serum levels of thromboxane B2 (TxB2). All eight untreated dogs died within 1 week of renal failure, and the nine treated dogs demonstrated transient renal failure, with a significant difference (P less than 0.001) being found between the two groups. A significant difference (P less than 0.001) in TxB2 levels was found in the untreated dogs before and after ischemia and between the two groups following reperfusion. Animals that are treated with SOD after the ischemic event has occurred but before reperfusion exhibit a favorable clinical course in terms of survival and renal function. Tx synthesis in the kidney can be blocked by the administration of SOD.
本研究旨在确定给予超氧化物歧化酶（SOD）是否能减轻缺血性肾损伤，以及氧自由基与血栓素（Tx）之间是否存在关联。在17只犬中，切除右肾并将左肾血管蒂夹闭75分钟。在再灌注前，用5毫克SOD冲洗缺血肾，并另外全身输注20毫克SOD。在缺血前和再灌注后从肾静脉采集血样，以测定血栓素B2（TxB2）的血清水平。所有8只未治疗的犬在肾衰竭1周内死亡，9只治疗的犬出现短暂肾衰竭，两组之间存在显著差异（P小于0.001）。在未治疗的犬中，缺血前后以及再灌注后两组之间TxB2水平存在显著差异（P小于0.001）。在缺血事件发生后但再灌注前用SOD治疗的动物在生存和肾功能方面表现出良好的临床过程。肾脏中Tx的合成可通过给予SOD来阻断。
