Kodentsova V M, Vrzhesinskaia O A, Alekseeva I A, Spirichev V B
Vopr Med Khim. 1991 Sep-Oct;37(5):76-9.
Various biochemical procedures were used in evaluation of vitamin B2 occurrence in 38 women. Highly specific procedures for evaluation of riboflavin in blood and urine (high performance liquid chromatography, titration with riboflavin-binding apoprotein, lumiflavin procedure) enabled to detect correlation between values of FAD-effect and excretion of riboflavin with urine as well as of total riboflavin content in erythrocytes, between concentration of free riboflavin in blood plasma and its excretion with urine. All the procedures studied were equally suitable for evaluation of vitamin B2 deficiency as shown by the data obtained as well as by similarity in values of low concentrations which corresponded to normal availability of the vitamin.
采用了多种生化方法来评估38名女性体内维生素B2的情况。用于评估血液和尿液中核黄素的高特异性方法(高效液相色谱法、用核黄素结合载脂蛋白滴定法、荧光黄素法)能够检测黄素腺嘌呤二核苷酸(FAD)效应值与尿中核黄素排泄量之间的相关性,以及红细胞中总核黄素含量与血浆中游离核黄素浓度及其尿排泄量之间的相关性。所研究的所有方法同样适用于评估维生素B2缺乏情况,所获数据以及与维生素正常可利用性相对应的低浓度值的相似性均表明了这一点。