Smirnov I V, Tseneva G Ia
Zh Mikrobiol Epidemiol Immunobiol. 1991 Sep(9):13-6.
Study of the cultivation properties of 82 enterobacterial strains has revealed that the colonies of virulent Y. enterocolitica (serovars O3, O9) and Y. pseudotuberculosis (serovar I) are temperature-sensitive. This sign, closely connected with the presence and expression of the virulence plasmid with a molecular weight of 44-48 MD, is not characteristic of other strains. Virulent Yersinia grown in nutrient agar for 48 hours at 37 degrees C form colonies which are smaller in diameter than those formed during cultivation at 26 degrees C (with the significance of differences t greater than or equal to 4), their diameter at 37 degrees C not exceeding 1.0 mm. The test for the determination of the temperature-sensitive morphology of Yersinia colonies, along with the tests for other virulence markers, is probably suitable for the detection of the causative agents of yersiniosis or pseudotuberculosis.
对82株肠杆菌菌株培养特性的研究表明,致病性小肠结肠炎耶尔森菌(血清型O3、O9)和假结核耶尔森菌(血清型I)的菌落对温度敏感。这一特征与分子量为44 - 48 MD的毒力质粒的存在和表达密切相关,其他菌株没有此特征。致病性耶尔森菌在营养琼脂中于37℃培养48小时形成的菌落,其直径比在26℃培养时形成的菌落小(差异显著性t大于或等于4),其在37℃时的直径不超过1.0毫米。检测耶尔森菌菌落温度敏感形态的试验,与其他毒力标志物检测试验一起,可能适用于检测耶尔森菌病或假结核病的病原体。