Smirnov I V, Gorokhov V I
Lab Delo. 1990(10):66-8.
A diagnostic agglutinating adsorbed rabbit serum with antibodies to Yersinia strains containing virulence plasmid with molecular mass of 40-50 mD was prepared. Trials of this serum in agglutination test on the glass with 69 Yersinia strains (Y. enterocolitica, Y. frederiksenii, Y. intermedia, Y. kristensenii, Y. pseudotuberculosis) and with 42 other Enterobacteriaceae strains have confirmed the specificity and sufficiently high activity of the agent in respect of virulent Yersinia strains. Experiments have demonstrated the possibility of using this serum in tests for the detection of yersiniasis agents and for rapid assessment of individual Yersinia clones in respect of the presence of plasmid with a molecular mass of 40-50 mD.
制备了一种诊断性凝集吸附兔血清,其含有针对携带分子量为40 - 50 mD毒力质粒的耶尔森菌菌株的抗体。用该血清对69株耶尔森菌菌株(小肠结肠炎耶尔森菌、费氏耶尔森菌、中间耶尔森菌、克氏耶尔森菌、假结核耶尔森菌)以及42株其他肠杆菌科菌株进行玻片凝集试验,证实了该制剂对有毒力的耶尔森菌菌株具有特异性和足够高的活性。实验证明了使用该血清检测耶尔森菌病病原体以及快速评估单个耶尔森菌克隆是否存在分子量为40 - 50 mD质粒的可能性。