Lacey N, Delaney S, Kavanagh K, Powell F C
Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland.
Br J Dermatol. 2007 Sep;157(3):474-81. doi: 10.1111/j.1365-2133.2007.08028.x. Epub 2007 Jun 26.
Patients with papulopustular rosacea have a higher density of Demodex folliculorum mites on their faces than normal subjects but the role, if any, of their mites in initiating inflammation is disputed. Selective antibiotics are effective in reducing the inflammatory changes of papulopustular rosacea, but their mode of action is unknown.
To investigate whether a D. folliculorum-related bacterium was capable of expressing antigens that could stimulate an inflammatory immune response in patients with rosacea.
A bacterium (Bacillus oleronius) was isolated from a D. folliculorum mite extracted from the face of a patient with papulopustular rosacea, and was investigated further.
This bacterium produced antigens capable of stimulating peripheral blood mononuclear cells proliferation in 16 of 22 (73%) patients with rosacea but only five of 17 (29%) control subjects (P = 0.0105). This antigenic preparation was fractionated into 70 subfractions and the proteins in each fraction were visualized by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Western blot analysis revealed the presence of two antigenic proteins of size 62 and 83 kDa in fractions when probing with sera from patients with rosacea. No immunoreactivity to these proteins was recorded when probing with sera from control patients. Two-dimensional electrophoretic separation was used to isolate these proteins and matrix-assisted laser desorption/ionization time-of-flight analysis was employed to identify the relevant peptides. The 62-kDa immunoreactive protein shared amino acid sequence homology with an enzyme involved in carbohydrate metabolism and signal transduction while the 83-kDa protein was similar to bacterial heat shock proteins.
Antigenic proteins related to a bacterium (B. oleronius), isolated from a D. folliculorum mite, have the potential to stimulate an inflammatory response in patients with papulopustular rosacea.
丘疹脓疱型酒渣鼻患者面部毛囊蠕形螨的密度高于正常受试者，但这些螨虫在引发炎症中所起的作用（如果有）存在争议。选择性抗生素可有效减轻丘疹脓疱型酒渣鼻的炎症变化，但其作用方式尚不清楚。
研究与毛囊蠕形螨相关的细菌是否能够表达可刺激酒渣鼻患者产生炎症免疫反应的抗原。
从一名丘疹脓疱型酒渣鼻患者面部提取的毛囊蠕形螨中分离出一种细菌（奥勒隆芽孢杆菌），并进行进一步研究。
该细菌产生的抗原能够刺激22例酒渣鼻患者中的16例（73%）外周血单核细胞增殖，而在17例对照受试者中只有5例（29%）出现这种情况（P = 0.0105）。将这种抗原制剂分离成70个亚组分，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳观察每个组分中的蛋白质。用酒渣鼻患者的血清进行免疫印迹分析时，发现某些组分中存在两种大小分别为62 kDa和83 kDa的抗原蛋白。用对照患者的血清进行检测时，未记录到对这些蛋白质的免疫反应性。采用二维电泳分离这些蛋白质，并利用基质辅助激光解吸/电离飞行时间分析来鉴定相关肽段。62 kDa的免疫反应性蛋白与一种参与碳水化合物代谢和信号转导的酶具有氨基酸序列同源性，而83 kDa的蛋白与细菌热休克蛋白相似。
从毛囊蠕形螨中分离出的一种细菌（奥勒隆芽孢杆菌）相关的抗原蛋白有可能刺激丘疹脓疱型酒渣鼻患者产生炎症反应。
