Department of Biology, National University of Ireland Maynooth, Co. Kildare, Ireland.
Br J Dermatol. 2012 Nov;167(5):1032-6. doi: 10.1111/j.1365-2133.2012.11114.x. Epub 2012 Sep 25.
Rosacea is a chronic inflammatory condition that affects the skin of the face and the eyes. Erythematotelangiectatic rosacea is characterized by flushing, oedema and telangiectasia. Patients with rosacea demonstrate elevated densities of Demodex mites in their skin compared with controls. A bacterium (Bacillus oleronius) isolated from Demodex mites from a patient with papulopustular rosacea has been demonstrated to produce antigenic proteins that may play a role in papulopustular and ocular rosacea.
To establish whether there was a correlation between the reactivity of sera from patients with erythematotelangiectatic rosacea to Bacillus antigens, and to characterize the proteins to which these patients showed reactivity.
Serum samples from patients with erythematotelangiectatic rosacea and controls were examined for reactivity to Bacillus proteins by Western blot analysis. Proteins to which the sera reacted were excised from gels, trypsin digested, and putative identities were assigned following liquid chromatography-mass spectrometry (LC-MS) analysis.
Eighty per cent (21/26) of patients with erythematotelangiectatic rosacea showed serum reactivity to the 62- and 83-kDa proteins of B. oleronius, compared with 40% (9/22) of controls (P = 0·004). The 62-kDa protein was characterized by LC-MS and showed homology to groEL chaperonin, which provokes a strong immune response in mammals. The 83-kDa protein showed homology to aconitate hydratase, of which expression is increased in bacteria under oxidative stress, and which is highly immunogenic.
The majority of patients with erythematotelangiectatic rosacea show serum reactivity to two proteins from B. oleronius, suggesting that this bacterium may play a role in the induction of this condition. The two proteins to which patient sera reacted were found to be similar to a heat shock protein and an enzyme involved in regulating the stress response of the bacterium.
酒渣鼻是一种影响面部和眼部皮肤的慢性炎症性疾病。红斑毛细血管扩张型酒渣鼻的特征是潮红、水肿和毛细血管扩张。与对照组相比，酒渣鼻患者的皮肤中蠕形螨密度升高。从患有脓疱性酒渣鼻的患者的蠕形螨中分离出的一种细菌（奥利龙芽孢杆菌）已被证明能产生抗原性蛋白，这些蛋白可能在脓疱性和眼部酒渣鼻中发挥作用。
确定红斑毛细血管扩张型酒渣鼻患者血清对芽孢杆菌抗原的反应性是否存在相关性，并对这些患者表现出反应性的蛋白进行特征描述。
通过 Western blot 分析检测红斑毛细血管扩张型酒渣鼻患者和对照组的血清对芽孢杆菌蛋白的反应性。从凝胶中切下与血清反应的蛋白，用胰蛋白酶消化，然后通过液相色谱-质谱（LC-MS）分析确定其可能的身份。
80%（21/26）的红斑毛细血管扩张型酒渣鼻患者血清对 B. oleronius 的 62-和 83-kDa 蛋白有反应，而对照组为 40%（9/22）（P=0·004）。62-kDa 蛋白通过 LC-MS 进行了鉴定，与哺乳动物中引起强烈免疫反应的 groEL 分子伴侣同源。83-kDa 蛋白与顺乌头酸水合酶同源，该酶在细菌受到氧化应激时表达增加，且具有高度免疫原性。
大多数红斑毛细血管扩张型酒渣鼻患者的血清对 B. oleronius 的两种蛋白有反应，这表明这种细菌可能在诱导这种疾病中发挥作用。患者血清反应的两种蛋白与热休克蛋白和一种参与调节细菌应激反应的酶相似。
