结核分枝杆菌中pncA基因突变、吡嗪酰胺酶活性与吡嗪酰胺药敏试验之间的关联
Association between pncA gene mutations, pyrazinamidase activity, and pyrazinamide susceptibility testing in Mycobacterium tuberculosis.
作者信息
Aono Akio, Chikamatsu Kinuyo, Yamada Hiroyuki, Kato Tomoko, Mitarai Satoshi
机构信息
Department of Mycobacterium Reference and Research, The Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Kiyose, Japan
Department of Mycobacterium Reference and Research, The Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Kiyose, Japan.
出版信息
Antimicrob Agents Chemother. 2014 Aug;58(8):4928-30. doi: 10.1128/AAC.02394-14. Epub 2014 May 27.
Abstract
We determined MICs for, confirmed the presence of pncA mutations in, and performed pyrazinamidase testing on colonies (subclones) obtained from seven isolates that exhibited differential pyrazinamide (PZA) susceptibility. Six of the seven strains were found to exhibit characteristics resulting from the mixture of strains possessing different properties. In addition, our analysis revealed large pncA-spanning deletions (1,565 bp, 4,475 bp, and 6,258 bp) in three strains that showed high PZA resistance.
摘要
我们测定了七株对吡嗪酰胺（PZA）敏感性不同的分离株所获得的菌落（亚克隆）的最低抑菌浓度（MIC），确认了其中pncA突变的存在，并对其进行了吡嗪酰胺酶测试。七株菌株中有六株表现出具有不同特性的菌株混合所产生的特征。此外，我们的分析揭示了三株对PZA高度耐药的菌株中存在跨越pncA的大片段缺失（分别为1,565 bp、4,475 bp和6,258 bp）。
相似文献
1
Association between pncA gene mutations, pyrazinamidase activity, and pyrazinamide susceptibility testing in Mycobacterium tuberculosis.结核分枝杆菌中pncA基因突变、吡嗪酰胺酶活性与吡嗪酰胺药敏试验之间的关联
Antimicrob Agents Chemother. 2014 Aug;58(8):4928-30. doi: 10.1128/AAC.02394-14. Epub 2014 May 27.
2
Surveillance of pyrazinamide susceptibility among multidrug-resistant Mycobacterium tuberculosis isolates from Siriraj Hospital, Thailand.泰国诗里拉吉医院耐多药结核分枝杆菌分离株中吡嗪酰胺敏感性监测。
BMC Microbiol. 2010 Aug 20;10:223. doi: 10.1186/1471-2180-10-223.
3
pncA mutations as a major mechanism of pyrazinamide resistance in Mycobacterium tuberculosis: spread of a monoresistant strain in Quebec, Canada.pncA突变作为结核分枝杆菌对吡嗪酰胺耐药的主要机制：单一耐药菌株在加拿大魁北克的传播
Antimicrob Agents Chemother. 2000 Mar;44(3):528-32. doi: 10.1128/AAC.44.3.528-532.2000.
4
Phenotypic and genotypic characterization of pyrazinamide resistance among multidrug-resistant Mycobacterium tuberculosis clinical isolates in Hangzhou, China.中国杭州耐多药结核分枝杆菌临床分离株中吡嗪酰胺耐药的表型和基因型特征。
Clin Microbiol Infect. 2018 Sep;24(9):1016.e1-1016.e5. doi: 10.1016/j.cmi.2017.12.012. Epub 2017 Dec 26.
5
Mycobacterium tuberculosis pyrazinamide resistance determinants: a multicenter study.结核分枝杆菌吡嗪酰胺耐药决定因素：一项多中心研究。
mBio. 2014 Oct 21;5(5):e01819-14. doi: 10.1128/mBio.01819-14.
6
Pyrazinamide resistance and mutations in pncA among isolates of Mycobacterium tuberculosis from Khyber Pakhtunkhwa, Pakistan.巴基斯坦开伯尔-普赫图赫瓦省结核分枝杆菌分离株中吡嗪酰胺耐药性及 pncA 基因突变。
BMC Infect Dis. 2019 Feb 6;19(1):116. doi: 10.1186/s12879-019-3764-2.
7
Relationship between pyrazinamide resistance, loss of pyrazinamidase activity, and mutations in the pncA locus in multidrug-resistant clinical isolates of Mycobacterium tuberculosis.耐多药结核分枝杆菌临床分离株中吡嗪酰胺耐药性、吡嗪酰胺酶活性丧失与pncA基因座突变之间的关系
Antimicrob Agents Chemother. 1999 Sep;43(9):2317-9. doi: 10.1128/AAC.43.9.2317.
8
Reevaluation of the critical concentration for drug susceptibility testing of Mycobacterium tuberculosis against pyrazinamide using wild-type MIC distributions and pncA gene sequencing.使用野生型 MIC 分布和 pncA 基因测序重新评估结核分枝杆菌对吡嗪酰胺药敏试验的临界浓度。
Antimicrob Agents Chemother. 2012 Mar;56(3):1253-7. doi: 10.1128/AAC.05894-11. Epub 2011 Dec 27.
9
Pyrazinamide resistance-conferring mutations in pncA and the transmission of multidrug resistant TB in Georgia.pncA基因中赋予吡嗪酰胺耐药性的突变与格鲁吉亚耐多药结核病的传播
BMC Infect Dis. 2017 Jul 12;17(1):491. doi: 10.1186/s12879-017-2594-3.
10
[Characteristics of pncA gene in multidrug-resistant Mycobacterium tuberculosis isolates and its correlation with drug resistance to pyrazinamide].耐多药结核分枝杆菌分离株中pncA基因的特征及其与吡嗪酰胺耐药性的相关性
Zhonghua Yu Fang Yi Xue Za Zhi. 2012 May;46(5):436-9.
引用本文的文献
1
JIB-04, an inhibitor of Jumonji histone demethylase as a potent antitubercular agent against Mycobacterium tuberculosis.JIB-04，一种组蛋白去甲基化酶 Jumonji 的抑制剂，作为一种有效的抗结核药物，针对结核分枝杆菌。
Arch Microbiol. 2024 Nov 19;206(12):470. doi: 10.1007/s00203-024-04197-9.
2
Pyrazinamide-resistant Tuberculosis Obscured From Common Targeted Molecular Diagnostics.吡嗪酰胺耐药结核病被常见的靶向分子诊断所掩盖。
Drug Resist Updat. 2023 May;68:100959. doi: 10.1016/j.drup.2023.100959. Epub 2023 Apr 6.
3
Mutations Associated with Pyrazinamide Resistance in Mycobacterium tuberculosis: A Review and Update.结核分枝杆菌吡嗪酰胺耐药相关突变：综述与更新。
Curr Microbiol. 2022 Oct 8;79(11):348. doi: 10.1007/s00284-022-03032-y.
4
Detection of Mutations in in Clinical Isolates from Nepal in Association with Pyrazinamide Resistance.尼泊尔临床分离株中与吡嗪酰胺耐药相关的突变检测
Curr Issues Mol Biol. 2022 Sep 8;44(9):4132-4141. doi: 10.3390/cimb44090283.
5
Pyrazinamide Resistance and pncA Mutation Profiles in Multidrug Resistant Mycobacterium Tuberculosis.耐多药结核分枝杆菌中的吡嗪酰胺耐药性及pncA基因突变情况
Infect Drug Resist. 2022 Aug 30;15:4985-4994. doi: 10.2147/IDR.S368444. eCollection 2022.
6
Characterization of Mutations and Prediction of PZA Resistance in Clinical Isolates From Chongqing, China.中国重庆临床分离株中突变的特征分析及吡嗪酰胺耐药性预测
Front Microbiol. 2021 Jan 11;11:594171. doi: 10.3389/fmicb.2020.594171. eCollection 2020.
7
Prediction of Mycobacterium tuberculosis pyrazinamidase function based on structural stability, physicochemical and geometrical descriptors.基于结构稳定性、物理化学和几何描述符预测结核分枝杆菌吡嗪酰胺酶的功能。
PLoS One. 2020 Jul 31;15(7):e0235643. doi: 10.1371/journal.pone.0235643. eCollection 2020.
8
Marine Natural Products and Drug Resistance in Latent Tuberculosis.海洋天然产物与潜伏结核的耐药性
Mar Drugs. 2019 Sep 26;17(10):549. doi: 10.3390/md17100549.
9
Molecular analysis of pyrazinamide resistance in Mycobacterium tuberculosis in Vietnam highlights the high rate of pyrazinamide resistance-associated mutations in clinical isolates.越南结核分枝杆菌中吡嗪酰胺耐药的分子分析凸显了临床分离株中吡嗪酰胺耐药相关突变的高发生率。
Emerg Microbes Infect. 2017 Oct 11;6(10):e86. doi: 10.1038/emi.2017.73.
10
A Multinational Analysis of Mutations and Heterogeneity in PZase, RpsA, and PanD Associated with Pyrazinamide Resistance in M/XDR Mycobacterium tuberculosis.跨国分析与吡嗪酰胺耐药相关的 PZase、RpsA 和 PanD 中的突变和异质性在 M/XDR 结核分枝杆菌中的作用。
Sci Rep. 2017 Jun 19;7(1):3790. doi: 10.1038/s41598-017-03452-y.
本文引用的文献
1
pncA gene expression and prediction factors on pyrazinamide resistance in Mycobacterium tuberculosis.结核分枝杆菌 pncA 基因表达与吡嗪酰胺耐药相关预测因素研究。
Tuberculosis (Edinb). 2013 Sep;93(5):515-22. doi: 10.1016/j.tube.2013.03.005. Epub 2013 Jul 16.
2
Reevaluation of the critical concentration for drug susceptibility testing of Mycobacterium tuberculosis against pyrazinamide using wild-type MIC distributions and pncA gene sequencing.使用野生型 MIC 分布和 pncA 基因测序重新评估结核分枝杆菌对吡嗪酰胺药敏试验的临界浓度。
Antimicrob Agents Chemother. 2012 Mar;56(3):1253-7. doi: 10.1128/AAC.05894-11. Epub 2011 Dec 27.
3
Pyrazinamide inhibits trans-translation in Mycobacterium tuberculosis.吡嗪酰胺抑制结核分枝杆菌中的转译-转译。
Science. 2011 Sep 16;333(6049):1630-2. doi: 10.1126/science.1208813. Epub 2011 Aug 11.
4
Development and evaluation of a line probe assay for rapid identification of pncA mutations in pyrazinamide-resistant mycobacterium tuberculosis strains.用于快速鉴定耐吡嗪酰胺结核分枝杆菌菌株中pncA基因突变的线性探针检测法的开发与评估
J Clin Microbiol. 2007 Sep;45(9):2802-7. doi: 10.1128/JCM.00352-07. Epub 2007 Jun 27.
5
Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis.结核分枝杆菌优化的分枝杆菌插入重复单位-可变数目串联重复序列分型标准化方案。
J Clin Microbiol. 2006 Dec;44(12):4498-510. doi: 10.1128/JCM.01392-06. Epub 2006 Sep 27.
6
Frequency and implications of pyrazinamide resistance in managing previously treated tuberculosis patients.既往接受治疗的结核病患者中吡嗪酰胺耐药的频率及其影响
Int J Tuberc Lung Dis. 2006 Jul;10(7):802-7.
7
Activation of pyrazinamide and nicotinamide in acidic environments in vitro.吡嗪酰胺和烟酰胺在体外酸性环境中的活化作用。
Am Rev Tuberc. 1954 Oct;70(4):748-54. doi: 10.1164/art.1954.70.4.748.
8
Evaluation of BACTEC MGIT 960 PZA medium for susceptibility testing of Mycobacterium tuberculosis to pyrazinamide (PZA): compared with the results of pyrazinamidase assay and Kyokuto PZA test.评估BACTEC MGIT 960吡嗪酰胺（PZA）培养基用于结核分枝杆菌对吡嗪酰胺（PZA）药敏试验：与吡嗪酰胺酶测定结果和京都PZA试验结果的比较
Diagn Microbiol Infect Dis. 2002 Dec;44(4):347-52. doi: 10.1016/s0732-8893(02)00471-6.
9
Conditions that may affect the results of susceptibility testing of Mycobacterium tuberculosis to pyrazinamide.可能影响结核分枝杆菌对吡嗪酰胺药敏试验结果的情况。
J Med Microbiol. 2002 Jan;51(1):42-49. doi: 10.1099/0022-1317-51-1-42.
10
pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis isolates from northwestern Russia.俄罗斯西北部耐吡嗪酰胺结核分枝杆菌分离株中的pncA基因突变
Antimicrob Agents Chemother. 1999 Jul;43(7):1764-6. doi: 10.1128/AAC.43.7.1764.
Zotero 插件