通过电学测量对储存全血进行生理质量评估。

Physiological quality assessment of stored whole blood by means of electrical measurements.

作者信息

Ulgen Y, Sezdi M

机构信息

Institute of Biomedical Engineering, Bogazici University, 34342 Bebek-Istanbul, Turkey.

出版信息

Med Biol Eng Comput. 2007 Jul;45(7):653-60. doi: 10.1007/s11517-007-0206-x. Epub 2007 Jun 28.

DOI:10.1007/s11517-007-0206-x

PMID:17597330

Abstract

The physiological parameters of blood such as extracellular Na(+), K(+), Cl(-), pH, 2,3-DPG and ATP and the complex electrical impedance were measured using whole blood samples from 31 male donors (21 donors form the training set and ten donors were used for testing), on the 0th, 10th and 21st day of blood bank storage. During storage, while the extracellular fluid resistance (R(e)) and the intracellular fluid resistance (R (i)) decreased progressively with time, the effective cell membrane capacitance (C(m)) has increased. Blood bank storage resulted in a rise in K(+) and a fall in Na(+), Cl(-), pH, 2,3-DPG and ATP. Accordingly, all electrical parameters correlated with Na(+), K(+), Cl(-), pH and ATP, at varying levels. By applying the multi-regression analysis, it was demonstrated that R (i), R (e) and especially C (m) were appropriate for the assessment of Na(+), K(+), Cl(-), pH and ATP until the 21st day of storage.

摘要

使用来自31名男性献血者（21名献血者组成训练集，10名献血者用于测试）的全血样本，在血库储存的第0天、第10天和第21天，测量血液的生理参数，如细胞外Na(+)、K(+)、Cl(-)、pH、2,3 - 二磷酸甘油酸（2,3 - DPG）和三磷酸腺苷（ATP）以及复电阻抗。在储存期间，虽然细胞外液电阻（R(e)）和细胞内液电阻（R(i)）随时间逐渐降低，但有效细胞膜电容（C(m)）却增加了。血库储存导致K(+)升高，而Na(+)、Cl(-)、pH、2,3 - DPG和ATP降低。因此，所有电学参数在不同程度上与Na(+)、K(+)、Cl(-)、pH和ATP相关。通过多元回归分析表明，直到储存第21天，R(i)、R(e)尤其是C(m)适用于评估Na(+)、K(+)、Cl(-)、pH和ATP。