Ho Thien, Wang Hui, Pallett Denise, Dalmay Tamas
NERC/Centre for Ecology and Hydrology Oxford, Oxford, UK.
FEBS Lett. 2007 Jul 10;581(17):3267-72. doi: 10.1016/j.febslet.2007.06.022. Epub 2007 Jun 21.
Small interfering (si)RNAs isolated from Brassica juncea leaves infected by Turnip mosaic virus (TuMV) were characterized by cloning and sequencing. The TuMV siRNA population was dominated by 21 and 22-nt long species originated mainly from the same siRNA hotspots, indicating operational similarity between the plant Dicer-like (DCL) enzymes. Robust GC bias was observed for TuMV siRNAs versus the virus genome, indicating that DCL was more likely to target GC-rich regions. Furthermore, dicot micro-(mi)RNAs displayed higher GC% than their DCL1 substrate RNAs, implicating that the GC bias may be ancient, therefore may be important for the RNAi technology.
通过克隆和测序对从感染芜菁花叶病毒（TuMV）的芥菜叶片中分离出的小干扰（si）RNA进行了表征。TuMV siRNA群体主要由长度为21和22个核苷酸的物种组成，这些物种主要起源于相同的siRNA热点，这表明植物类Dicer（DCL）酶之间存在操作上的相似性。观察到TuMV siRNA相对于病毒基因组存在明显的GC偏好，这表明DCL更有可能靶向富含GC的区域。此外，双子叶植物的微小（mi）RNA比其DCL1底物RNA显示出更高的GC百分比，这意味着GC偏好可能是古老的，因此可能对RNA干扰技术很重要。
