Erdogan M, Karadeniz M, Berdeli A, Tamsel S, Ertan Y, Uluer H, Yilmaz C, Tuzun M, Kabalak T, Ozgen A G
Department of Endocrinology and Metabolism Disease, Ege University Medical School, Bornova, Izmir, Turkey.
J Endocrinol Invest. 2007 May;30(5):411-6. doi: 10.1007/BF03346319.
Fas ligand (FasL) is an apoptotic agent and a member of tumor necrosis factor (TNF) family. FasL exists in cytotoxic T lymphocyte (CTL) and natural killer (NK) cells, and it is increased in tumor cell membrane. On the contrary, CTL and NK are bound to Fas on the surfaces of cell membrane; this triggers apoptosis in cytotoxic cells and leads to their death. This system plays an important role in eliminating viral infections and cancer cells. Malfunction of this system results in the development and spread of the malignancy. This study aims at evaluating the influence of Fas and FasL gene polymorphism in papillary thyroid cancer (PTC) in the Turkish population.
Forty-five patients with PTC and 100 healthy controls were included in this study. The diagnosis of PTC was confirmed by histopathologic examination after surgery. The evaluation of genotype for Fas 670 A/G and FasL 843 C/T gene polymorphism was performed using the PCR-restriction fragment length polymorphism (RFLP) method.
The evaluation of Fas/FasL genotype and gene allele frequency did not show statistically significant differences between the patient and control group (p>0.05). In addition, the univariate analysis did not reveal a statistically significant relationship between the size of the nodule and the Fas/FasL gene polymorphism in patients with PTC.
As in other types of malignancy, genetic factors in the pathogenesis of PTC may also show changes in different populations. Fas/FasL gene polymorphysms are possible that different mechanisms function in apoptosis balance in PTC development.
Fas配体（FasL）是一种凋亡因子，属于肿瘤坏死因子（TNF）家族成员。FasL存在于细胞毒性T淋巴细胞（CTL）和自然杀伤（NK）细胞中，且在肿瘤细胞膜上表达增加。相反，CTL和NK细胞与细胞膜表面的Fas结合，这会触发细胞毒性细胞的凋亡并导致其死亡。该系统在清除病毒感染和癌细胞方面发挥着重要作用。该系统功能失调会导致恶性肿瘤的发生和扩散。本研究旨在评估土耳其人群中Fas和FasL基因多态性对甲状腺乳头状癌（PTC）的影响。
本研究纳入了45例PTC患者和100例健康对照。PTC的诊断通过手术后的组织病理学检查得以证实。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对Fas 670 A/G和FasL 843 C/T基因多态性进行基因分型评估。
Fas/FasL基因分型及基因等位基因频率评估显示，患者组与对照组之间无统计学显著差异（p>0.05）。此外，单因素分析未揭示PTC患者结节大小与Fas/FasL基因多态性之间存在统计学显著关系。
与其他类型的恶性肿瘤一样，PTC发病机制中的遗传因素在不同人群中可能也会有所变化。Fas/FasL基因多态性可能在PTC发生过程中的凋亡平衡中发挥不同作用机制。
