Erdogan M, Karadeniz M, Ozbek M, Ozgen A G, Berdeli A
Department of Endocrinology and Metabolism Disease, Ege University Medical School, Bornova, Izmir, Turkey.
J Endocrinol Invest. 2008 Sep;31(9):750-4. doi: 10.1007/BF03349252.
Interleukin-10 (IL-10) is a major anti-inflammatory cytokine that plays a crucial role in the regulation of the immune system. Chronic inflammation has been reported to be a risk factor for thyroid neoplasia. The propensity to mount an inflammatory response is modified by germ line variation in cytokine and other inflammation-related genes. We hypothesized that a proinflammatory genotype would be positively associated with thyroid cancer. We aimed to evaluate the relation between the genotypic and allelic frequencies of the IL-10(-1082 G/A), IL-10(-592 A/C), and IL-10(-819 C/T) polymorphisms, and their association with the risk of developing papillary thyroid cancer (PTC) in the Turkish population.
Forty-two patients with PTC and 113 healthy controls were included in this study. The diagnosis of PTC was confirmed by histopathologic examination after surgery. The evaluation of genotype for IL-10 gene polymorphism was performed using PCR-restriction fragment length polymorphism method.
Statistically significant difference IL-10(-1082 G/A) gene polymorphism was determined between 2 (PTC and control) groups. No difference was determined with respect to IL-10(-592 A/C) and IL-10(-819 C/T) gene polymorphisms, and IL-10(-1082 G/A), IL-10(-592 A/C), and IL-10(-819 C/T) allele frequencies of participating between the control group and the patients with PTC (p>0.05).
The polymorphism of IL-10(-1082 G/A) gene was significantly associated with the occurrence of PTC. Such studies will contribute significantly to our understanding of the biological role of IL-10(-1082 G/A) gene polymorphism in PTC development. In conclusion, IL-10(-1082 G/A) gene polymorphism may affect the survival of papillary thyroid carcinoma.
白细胞介素-10（IL-10）是一种主要的抗炎细胞因子，在免疫系统调节中起关键作用。据报道，慢性炎症是甲状腺肿瘤形成的一个危险因素。细胞因子和其他炎症相关基因的种系变异会改变引发炎症反应的倾向。我们假设促炎基因型与甲状腺癌呈正相关。我们旨在评估IL-10（-1082 G/A）、IL-10（-592 A/C）和IL-10（-819 C/T）多态性的基因型和等位基因频率之间的关系，以及它们与土耳其人群中发生乳头状甲状腺癌（PTC）风险的关联。
本研究纳入了42例PTC患者和113例健康对照。PTC的诊断通过手术后的组织病理学检查得以证实。使用聚合酶链反应-限制性片段长度多态性方法对IL-10基因多态性的基因型进行评估。
在2组（PTC组和对照组）之间确定了IL-10（-1082 G/A）基因多态性存在统计学显著差异。在IL-10（-592 A/C）和IL-10（-819 C/T）基因多态性方面以及对照组与PTC患者之间参与的IL-10（-1082 G/A）、IL-10（-592 A/C）和IL-10（-819 C/T）等位基因频率方面均未确定差异（p>0.05）。
IL-10（-1082 G/A）基因多态性与PTC的发生显著相关。此类研究将对我们理解IL-10（-1082 G/A）基因多态性在PTC发生发展中的生物学作用有显著贡献。总之，IL-10（-1082 G/A）基因多态性可能影响乳头状甲状腺癌的生存。
