Eun Young Gyu, Lee Young Chan, Kim Su Kang, Chung Joo-Ho, Kwon Kee Hwan, Park Il Seok
Department of Otolaryngology-Head and Neck Surgery, School of Medicine, Kyung Hee University, Seoul, Republic of Korea.
Kohwang Medical Research Institute, Kyung Hee University, Seoul, Republic of Korea.
Auris Nasus Larynx. 2015 Aug;42(4):326-31. doi: 10.1016/j.anl.2015.02.001. Epub 2015 Mar 29.
Fas is the prototypic representative of the death receptor subgroup of the tumor necrosis factor (TNF) receptor family. Recently, single nucleotide polymorphisms (SNPs) of the Fas or Fas ligand (FasL) genes have been shown to be associated with an increased risk of several cancers and with the prognosis of several cancers. The objective of this study was to evaluate the association between the SNPs of the Fas and FasL genes and papillary thyroid cancer (PTC) and to assess the relationship between these SNPs and the clinicopathological characteristics of PTC.
Five SNPs located within the two genes of Fas and FasL were genotyped using direct sequencing in 94 patients with PTC and 364 healthy controls. Genetic data were analyzed using commercially available software. And, the statistical analyses were performed according to clinicopathologic characteristics of PTC.
Genotyping analysis demonstrated that the intron SNP (rs1571013), promoter SNP (rs1800682) and 3'-UTR SNP (rs1468063) of Fas were significantly associated with the development of PTC. We also detected a significant difference between patients with PTC and healthy controls with respect to Fas gene allele frequencies. Furthermore, we found that the 3'-UTR SNP (rs1468063) of Fas was associated with the multifocality of cancer [dominant model, OR 0.28, p=0.028; log-additive model, OR 0.43, p=0.033].
We observed a significant association between SNPs of the Fas gene and the development of PTC. In addition, there was a significant association between a Fas SNP and the multifocality of PTC.
Fas是肿瘤坏死因子（TNF）受体家族死亡受体亚组的典型代表。最近，Fas或Fas配体（FasL）基因的单核苷酸多态性（SNP）已被证明与多种癌症的风险增加以及多种癌症的预后相关。本研究的目的是评估Fas和FasL基因的SNP与甲状腺乳头状癌（PTC）之间的关联，并评估这些SNP与PTC临床病理特征之间的关系。
采用直接测序法对94例PTC患者和364例健康对照者进行Fas和FasL两个基因内的5个SNP基因分型。使用商用软件分析遗传数据。并且，根据PTC的临床病理特征进行统计分析。
基因分型分析表明，Fas的内含子SNP（rs1571013）、启动子SNP（rs1800682）和3'-UTR SNP（rs1468063）与PTC的发生显著相关。我们还检测到PTC患者与健康对照者在Fas基因等位基因频率方面存在显著差异。此外，我们发现Fas的3'-UTR SNP（rs1468063）与癌症的多灶性相关[显性模型，OR 0.28，p = 0.028；对数加性模型，OR 0.43，p = 0.033]。
我们观察到Fas基因的SNP与PTC的发生之间存在显著关联。此外，Fas的一个SNP与PTC的多灶性之间存在显著关联。
